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Sodium percarbonate and peracetic acid application affects the diagnostics of Ichthyophthirius multifiliis in rainbow trout (Oncorhynchus mykiss) via real-time PCR

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000144" target="_blank" >RIV/00027162:_____/23:N0000144 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://delegate-reg.co.uk/eafp2023" target="_blank" >https://delegate-reg.co.uk/eafp2023</a>

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Sodium percarbonate and peracetic acid application affects the diagnostics of Ichthyophthirius multifiliis in rainbow trout (Oncorhynchus mykiss) via real-time PCR

  • Popis výsledku v původním jazyce

    Pojezdal, L., Minarova, H., Matejickova K., Motlova J., Sodium percarbonate and peracetic acid application affects the diagnostics of Ichthyophthirius multifiliis in rainbow trout (Oncorhynchus mykiss) via real-time PCR. 21st International Conference on Diseases of Fish and Shellfish, Abstract Book, European Association of Fish Pathologists, Aberdeen, UK, 11.–14. 9. 2023, p. 186. Introduction: Dietary immunostimulation represents a valuable method of disease prevention, especially in intensive aquaculture, where frequent exposure of fish to stress may be the cause of immunosuppression. Selenium and zinc are known to have an immunomodulatory effect; however, these effects have not been thoroughly investigated in fish. Methodology: Selenium (1.5 and 3.0 mg/kg), zinc (15 and 30 mg/kg), and a combination of their higher concentrations (selenium 3.0 mg/kg and zinc 30 mg/kg) were administered to rainbow trout (Oncorhynchus mykiss) in feed for 11 weeks. At week 6, half of the fish from the five selenium/zinc groups and from the control group were experimentally infected with Aeromonas salmonicida. Samples of the head kidney were taken after euthanasia of the fish at weeks 6 and 11 (before and after infection), and a non-specific mitogen-driven, as well as specific antigen-driven lymphocyte proliferation assay, was performed. Results: With non-specific stimulation, the highest proliferative activity was observed in the groups fed dietary selenium, while the lowest proliferation levels were detected in the control group. The results were similar before and after the experimental infection with A. salmonicida, with the highest non-specific immune response observed first in the group fed the low concentration of dietary selenium and, after the infection, in the group fed the high concentration of dietary selenium. However, with specific stimulation after infection, a significant increase in proliferation levels compared to the control group was observed in the fish fed dietary zinc. Conclusions: While dietary selenium seemed to induce an increase in the non-specific immune response, dietary zinc was proven to provide a significant enhancement of the specific immune response in rainbow trout. More research to confirm these effects in response to different stimuli, and a complex examination are necessary to prove the positive impact of dietary selenium and zinc on fish health and their suitability for use in aquaculture.

  • Název v anglickém jazyce

    Sodium percarbonate and peracetic acid application affects the diagnostics of Ichthyophthirius multifiliis in rainbow trout (Oncorhynchus mykiss) via real-time PCR

  • Popis výsledku anglicky

    Pojezdal, L., Minarova, H., Matejickova K., Motlova J., Sodium percarbonate and peracetic acid application affects the diagnostics of Ichthyophthirius multifiliis in rainbow trout (Oncorhynchus mykiss) via real-time PCR. 21st International Conference on Diseases of Fish and Shellfish, Abstract Book, European Association of Fish Pathologists, Aberdeen, UK, 11.–14. 9. 2023, p. 186. Introduction: Dietary immunostimulation represents a valuable method of disease prevention, especially in intensive aquaculture, where frequent exposure of fish to stress may be the cause of immunosuppression. Selenium and zinc are known to have an immunomodulatory effect; however, these effects have not been thoroughly investigated in fish. Methodology: Selenium (1.5 and 3.0 mg/kg), zinc (15 and 30 mg/kg), and a combination of their higher concentrations (selenium 3.0 mg/kg and zinc 30 mg/kg) were administered to rainbow trout (Oncorhynchus mykiss) in feed for 11 weeks. At week 6, half of the fish from the five selenium/zinc groups and from the control group were experimentally infected with Aeromonas salmonicida. Samples of the head kidney were taken after euthanasia of the fish at weeks 6 and 11 (before and after infection), and a non-specific mitogen-driven, as well as specific antigen-driven lymphocyte proliferation assay, was performed. Results: With non-specific stimulation, the highest proliferative activity was observed in the groups fed dietary selenium, while the lowest proliferation levels were detected in the control group. The results were similar before and after the experimental infection with A. salmonicida, with the highest non-specific immune response observed first in the group fed the low concentration of dietary selenium and, after the infection, in the group fed the high concentration of dietary selenium. However, with specific stimulation after infection, a significant increase in proliferation levels compared to the control group was observed in the fish fed dietary zinc. Conclusions: While dietary selenium seemed to induce an increase in the non-specific immune response, dietary zinc was proven to provide a significant enhancement of the specific immune response in rainbow trout. More research to confirm these effects in response to different stimuli, and a complex examination are necessary to prove the positive impact of dietary selenium and zinc on fish health and their suitability for use in aquaculture.

Klasifikace

  • Druh

    O - Ostatní výsledky

  • CEP obor

  • OECD FORD obor

    40103 - Fishery

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/QK22010369" target="_blank" >QK22010369: Optimalizace řešení parazitárních onemocnění v chovech ryb se zaměřením na infekci Ichthyophthirius multifiliis u pstruha duhového</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2023

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů