Early onset of apc/c activity renders sac inefficient in mouse embryos

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000021" target="_blank" >RIV/00027162:_____/24:N0000021 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/24:00584848 RIV/00216224:14310/24:00135724

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fcell.2024.1355979/abstract" target="_blank" >https://www.frontiersin.org/articles/10.3389/fcell.2024.1355979/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fcell.2024.1355979" target="_blank" >10.3389/fcell.2024.1355979</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Early onset of apc/c activity renders sac inefficient in mouse embryos

Popis výsledku v původním jazyce

Control mechanisms of spindle assembly and chromosome segregaDon are vital for prevenDng aneuploidy during cell division. The mammalian germ cells and embryos are prone to chromosome segregaDon errors and resulDng aneuploidy is a major cause of terminaDon of development or severe developmental disorders. Here we focused on early mouse embryos, and using combinaDon of methods involving microinjecDon, immunodetecDon and confocal live cell imaging, we concentrated on the Spindle Assembly Checkpoint (SAC) and Anaphase PromoDng Complex/Cyclosome (APC/C). These are two important mechanisms cooperaDng during mitosis to ensure accurate chromosome segregaDon, and assessed their acDvity during the first two mitoses a[er ferDlizaDon. Our results showed, that in zygotes and 2-cell embryos, the SAC core protein Mad1 shows very low levels on kinetochores in comparison to oocytes and its interacDon with chromosomes is restricted to a short Dme interval a[er nuclear membrane disassembly (NEBD). Exposure of 2-cell embryos to low levels of spindle poison does not prevent anaphase, despite the spindle damage induced by the drug. And lastly, the APC/C is acDvated coincidentally with NEBD, before the spindle assembly compleDon. This early onset of APC/C acDvity, together with precocious relocalizaDon of Mad1 from chromosomes, prevents proper surveillance of spindle assembly by SAC. The results contribute to the understanding of the origin of aneuploidy in early embryos.

Název v anglickém jazyce

Early onset of apc/c activity renders sac inefficient in mouse embryos

Popis výsledku anglicky

Control mechanisms of spindle assembly and chromosome segregaDon are vital for prevenDng aneuploidy during cell division. The mammalian germ cells and embryos are prone to chromosome segregaDon errors and resulDng aneuploidy is a major cause of terminaDon of development or severe developmental disorders. Here we focused on early mouse embryos, and using combinaDon of methods involving microinjecDon, immunodetecDon and confocal live cell imaging, we concentrated on the Spindle Assembly Checkpoint (SAC) and Anaphase PromoDng Complex/Cyclosome (APC/C). These are two important mechanisms cooperaDng during mitosis to ensure accurate chromosome segregaDon, and assessed their acDvity during the first two mitoses a[er ferDlizaDon. Our results showed, that in zygotes and 2-cell embryos, the SAC core protein Mad1 shows very low levels on kinetochores in comparison to oocytes and its interacDon with chromosomes is restricted to a short Dme interval a[er nuclear membrane disassembly (NEBD). Exposure of 2-cell embryos to low levels of spindle poison does not prevent anaphase, despite the spindle damage induced by the drug. And lastly, the APC/C is acDvated coincidentally with NEBD, before the spindle assembly compleDon. This early onset of APC/C acDvity, together with precocious relocalizaDon of Mad1 from chromosomes, prevents proper surveillance of spindle assembly by SAC. The results contribute to the understanding of the origin of aneuploidy in early embryos.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40203 - Husbandry

Projekt

<a href="/cs/project/GA20-25850S" target="_blank" >GA20-25850S: Kontrola vstupu do anafáze během časného vývoje</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Cell and Developmental Biology

ISSN

2296-634X

e-ISSN

2296-634X

Svazek periodika

12

Číslo periodika v rámci svazku

2024

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

001190896200001

EID výsledku v databázi Scopus

2-s2.0-85188590577