Characterization of Streptococcus suis isolates collected from different animal species in the Czech Republic between 2018 and 2022

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000109" target="_blank" >RIV/00027162:_____/24:N0000109 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.eavld2024.org/wp-content/uploads/2024/10/Programma-DEFINITIVO-WEB.pdf" target="_blank" >https://www.eavld2024.org/wp-content/uploads/2024/10/Programma-DEFINITIVO-WEB.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Streptococcus suis isolates collected from different animal species in the Czech Republic between 2018 and 2022

Popis výsledku v původním jazyce

Introduction: Streptococcus suis is important pathogen primarily associated with pigs. Here we present a description of 622 isolates collected from different animal species between 2018 and 2022. Materials and methods: Blood agar plates were used for primoculture (22-48 hours at 37°C) of sectional materials or swabs. Suspected S. suis colonies were tested using biochemical test, rapid slide latex agglutination, and MALDI–TOF. Serotyping by multiplex PCR method and PCR-MLST typing were performed on DNA prepared by the boiling method. All isolates untypeable by PCR were tested with the co-agglutination test using antisera prepared via the immunisation of rabbits with reference strains (Zouharová et al. 2022). Results: The vast majority of isolates were collected from pigs (597 isolates, 95.98%), followed by isolates from cattle (20 isolates, 3.21%), geese (3 isolates, 0.48%), a dog (1 isolate, 0.16%) and a parrot (1 isolate, 0.16%). 22 different serotypes, including the so-called Suis-like serotypes, were identified. The most prevalent were untypeable isolates followed by serotype 7. Similar to serotypes, a wide spectrum of MLST types was identified, with the most prevalent being unknown sequence types. Discussion and Conclusion: Similar to other countries in Europe (Rieckmann et al. 2018), a wide diversity of S. suis isolates was found in the Czech Republic. Among clinically important serotypes, serotypes 2 and 7 were frequently detected in pathological material. This work was supported by grants RO0523 and TN02000017.

Název v anglickém jazyce

Characterization of Streptococcus suis isolates collected from different animal species in the Czech Republic between 2018 and 2022

Popis výsledku anglicky

Introduction: Streptococcus suis is important pathogen primarily associated with pigs. Here we present a description of 622 isolates collected from different animal species between 2018 and 2022. Materials and methods: Blood agar plates were used for primoculture (22-48 hours at 37°C) of sectional materials or swabs. Suspected S. suis colonies were tested using biochemical test, rapid slide latex agglutination, and MALDI–TOF. Serotyping by multiplex PCR method and PCR-MLST typing were performed on DNA prepared by the boiling method. All isolates untypeable by PCR were tested with the co-agglutination test using antisera prepared via the immunisation of rabbits with reference strains (Zouharová et al. 2022). Results: The vast majority of isolates were collected from pigs (597 isolates, 95.98%), followed by isolates from cattle (20 isolates, 3.21%), geese (3 isolates, 0.48%), a dog (1 isolate, 0.16%) and a parrot (1 isolate, 0.16%). 22 different serotypes, including the so-called Suis-like serotypes, were identified. The most prevalent were untypeable isolates followed by serotype 7. Similar to serotypes, a wide spectrum of MLST types was identified, with the most prevalent being unknown sequence types. Discussion and Conclusion: Similar to other countries in Europe (Rieckmann et al. 2018), a wide diversity of S. suis isolates was found in the Czech Republic. Among clinically important serotypes, serotypes 2 and 7 were frequently detected in pathological material. This work was supported by grants RO0523 and TN02000017.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/TN02000017" target="_blank" >TN02000017: Národní Centrum Biotechnologií ve Veterinární Medicíně</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů