Severe metabolic disturbance in fish diseased with carp edema virus infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000220" target="_blank" >RIV/00027162:_____/24:N0000220 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Severe metabolic disturbance in fish diseased with carp edema virus infection

Popis výsledku v původním jazyce

Pikula, J., Pojezdal, Ľ., Papežíková, I., Novotná, H., Mikulíková, I., Banďouchová, H., Blahová, J., Bednarska, M., Mareš, J., Palíková, M., 2024. Severe metabolic disturbance in fish diseased with carp edema virus infection. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 9. ISBN 978-80-7701-005-4. Significant mortalities associated with emerging viral diseases are challenging the economy of common carp aquaculture. As such, there is an increased need to disentangle how infected fish cope with progressive disease pathology and lose the ability for homeostatic maintenance of key physiological parameters. A natural carp edema virus (CEV) infection outbreak at a carp fish farm provided an opportunity to examine diseased and healthy carp in the same storage pond. thereby contributing to our better understanding of CEV disease pathophysiology. The disease status of fish was determined using PCR-based virus identification combined with analysis of gill pathology. Compared with healthy control carp, the blood chemistry profile of CEV-infected fish revealed major disruptions in electrolyte and acid-base balance (i.e., hyponatraemia, hypochloraemia, hyperphosphatemia, elevated pH, base excess, and anion gap and decreased partial dissolved carbon dioxide). In addition, we recorded hyperproteinaemia, hyperammonaemia, and decreased lactate along with increased creatinine, alkaline hyperalbuminaemia, hypotonic phosphatase, alanine aminotransferase, and aspartate aminotransferase. Red blood cell dehydration, endogenous associated hematology variables were also elevated. The multivariate pattern of responses for blood chemistry variables (driven by sodium, pl, partial dissolved carbon dioxide, ammonia, and albumin in the principal component analysis) clearly discriminated between CEV-infected and control carp. To conclude, we show that CEV infection in carp exerts complex adverse effects and results in severe metabolic disturbance due to the impaired gill respiratory and excretory functioning.

Název v anglickém jazyce

Severe metabolic disturbance in fish diseased with carp edema virus infection

Popis výsledku anglicky

Pikula, J., Pojezdal, Ľ., Papežíková, I., Novotná, H., Mikulíková, I., Banďouchová, H., Blahová, J., Bednarska, M., Mareš, J., Palíková, M., 2024. Severe metabolic disturbance in fish diseased with carp edema virus infection. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 9. ISBN 978-80-7701-005-4. Significant mortalities associated with emerging viral diseases are challenging the economy of common carp aquaculture. As such, there is an increased need to disentangle how infected fish cope with progressive disease pathology and lose the ability for homeostatic maintenance of key physiological parameters. A natural carp edema virus (CEV) infection outbreak at a carp fish farm provided an opportunity to examine diseased and healthy carp in the same storage pond. thereby contributing to our better understanding of CEV disease pathophysiology. The disease status of fish was determined using PCR-based virus identification combined with analysis of gill pathology. Compared with healthy control carp, the blood chemistry profile of CEV-infected fish revealed major disruptions in electrolyte and acid-base balance (i.e., hyponatraemia, hypochloraemia, hyperphosphatemia, elevated pH, base excess, and anion gap and decreased partial dissolved carbon dioxide). In addition, we recorded hyperproteinaemia, hyperammonaemia, and decreased lactate along with increased creatinine, alkaline hyperalbuminaemia, hypotonic phosphatase, alanine aminotransferase, and aspartate aminotransferase. Red blood cell dehydration, endogenous associated hematology variables were also elevated. The multivariate pattern of responses for blood chemistry variables (driven by sodium, pl, partial dissolved carbon dioxide, ammonia, and albumin in the principal component analysis) clearly discriminated between CEV-infected and control carp. To conclude, we show that CEV infection in carp exerts complex adverse effects and results in severe metabolic disturbance due to the impaired gill respiratory and excretory functioning.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů