Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000071" target="_blank" >RIV/00027162:_____/25:N0000071 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00140264

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12602-024-10259-7" target="_blank" >https://link.springer.com/article/10.1007/s12602-024-10259-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12602-024-10259-7" target="_blank" >10.1007/s12602-024-10259-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic

Popis výsledku v původním jazyce

Probiotics are important components for maintaining a healthy gut microbiota in pigs, especially in post-weaned piglets. Lactic acid bacteria derived from the gastrointestinal tract of wild boars can serve as a source of beneficial probiotic strains with suitable properties for use in pig husbandry. In the study, we analyzed and characterized 15 strains of Limosilactobacillus mucosae obtained from the gut contents of wild boars to assess their safety and beneficial properties as probiotic candidates. The strains were compared using pan genomic analysis with 49 L. mucosae strains obtained from the NCBI database. All isolated strains demonstrated safety by absence of transferrable antimicrobial resistance genes and hemolysin activity. Based on the presence of beneficial genes, five candidates with probiotic properties were selected and subjected to phenotypic profiling. Five selected isolates exhibited the ability to survive conditions mimicking passage through the host’s digestive tract, such as low pH and the presence of bile salts. Furthermore, five selected strains demonstrated the presence of multiple carbohydrate-active enzymes and the ability to utilize various carbohydrate substrates. These strains can enhance the digestibility of oligo- or polysaccharide substrates found in food or feed, specifically resistant starch, α-galactosides, cellobiose, gentiobiose, and arabinoxylans. Based on the results obtained, the L. mucosae isolates tested in the study appear to be promising candidates for use as probiotics in pigs.

Název v anglickém jazyce

Comparative Genome Analysis and Characterization of the Probiotic Properties of Lactic Acid Bacteria Isolated from the Gastrointestinal Tract of Wild Boars in the Czech Republic

Popis výsledku anglicky

Probiotics are important components for maintaining a healthy gut microbiota in pigs, especially in post-weaned piglets. Lactic acid bacteria derived from the gastrointestinal tract of wild boars can serve as a source of beneficial probiotic strains with suitable properties for use in pig husbandry. In the study, we analyzed and characterized 15 strains of Limosilactobacillus mucosae obtained from the gut contents of wild boars to assess their safety and beneficial properties as probiotic candidates. The strains were compared using pan genomic analysis with 49 L. mucosae strains obtained from the NCBI database. All isolated strains demonstrated safety by absence of transferrable antimicrobial resistance genes and hemolysin activity. Based on the presence of beneficial genes, five candidates with probiotic properties were selected and subjected to phenotypic profiling. Five selected isolates exhibited the ability to survive conditions mimicking passage through the host’s digestive tract, such as low pH and the presence of bile salts. Furthermore, five selected strains demonstrated the presence of multiple carbohydrate-active enzymes and the ability to utilize various carbohydrate substrates. These strains can enhance the digestibility of oligo- or polysaccharide substrates found in food or feed, specifically resistant starch, α-galactosides, cellobiose, gentiobiose, and arabinoxylans. Based on the results obtained, the L. mucosae isolates tested in the study appear to be promising candidates for use as probiotics in pigs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1910351" target="_blank" >QK1910351: Divoká a domácí prasata jako zdroj probiotických kultur a vliv technologických postupů přípravy a skladby synbiotického krmiva na jeho efektivitu a funkčnost</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Probiotics and Antimicrobial Proteins

ISSN

1867-1306

e-ISSN

1867-1314

Svazek periodika

17

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

19

Strana od-do

1820-1838

Kód UT WoS článku

001207122200002

EID výsledku v databázi Scopus

2-s2.0-85191091250