Development of a porcine model for two types of deep wound infections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000185" target="_blank" >RIV/00027162:_____/25:N0000185 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of a porcine model for two types of deep wound infections

Popis výsledku v původním jazyce

Multidrug resistance is a serious medical problem and represents a major challenge for the treatment of infectious diseases. There is an urgent need to develop new antimicrobial agents with diverse chemical structures and novel mechanisms of action to overcome resistance. For the implementation of new antimicrobial biomaterials and additives in human medicine, it is necessary to evaluate their potential not only in vitro but also in vivo. Porcine skin provides a comprehensive model for testing topical administration of antimicrobial biomaterials to simulate a real clinical situation – complicated skin and soft tissue infection (cSSTI). This poster presents the introduction of cSSTI caused by methicillin-resistant Staphylococcus aureus (MRSA) or Pseudomonas aeruginosa in fully immunocompetent pig models. Under general anesthesia, 10 full-thickness skin defects (5 x 5 cm) were surgically created on the dorsal surface of each pig after antiseptic preparation. Fascial incisions were made in the defects to induce complicated deep wound and soft tissue infection (fasciitis and myonecrosis) and to maintain the infection for the duration of the experiment. For wound infection, a bacterial inoculum of MRSA NRL/Atb 5922 strain (sequence type ST22) was diffusely applied to sterile gauze covering the skin defects in 10 pigs or the multidrug-resistant clinical strain P. aeruginosa FF2 (VIM-carbapenemase-producing) in 6 pigs. Subsequently, all wounds of each pig were covered with an occlusive dressing and sterile gauze, and finally the pigs were dressed in an elastic bandage. Analgesia was achieved with the use of meloxicam for three consecutive days. Preoperative antibiotic prophylaxis was used to reduce the risk of contaminating bacterial infections affecting the induced infections. On days 4, 7, 10 and 14, wounds were visually assessed and biopsies were taken for microbiological and histological examination as well as for gene expression. There was no significant deterioration in the overall health of the animals during the experimental period. The cSSTI infection model for both pathogens was effectively established and maintained for 14 days. Due to the immunocompetence of the model animals, all immunological responses to the infectious agent and wound healing processes are preserved.Acknowledgements: The work was supported by Ministry of Health of the Czech Republic, Grant Numbers NV19-05-00214, NU20-05-00166 and NU22-05-00475.

Název v anglickém jazyce

Development of a porcine model for two types of deep wound infections

Popis výsledku anglicky

Multidrug resistance is a serious medical problem and represents a major challenge for the treatment of infectious diseases. There is an urgent need to develop new antimicrobial agents with diverse chemical structures and novel mechanisms of action to overcome resistance. For the implementation of new antimicrobial biomaterials and additives in human medicine, it is necessary to evaluate their potential not only in vitro but also in vivo. Porcine skin provides a comprehensive model for testing topical administration of antimicrobial biomaterials to simulate a real clinical situation – complicated skin and soft tissue infection (cSSTI). This poster presents the introduction of cSSTI caused by methicillin-resistant Staphylococcus aureus (MRSA) or Pseudomonas aeruginosa in fully immunocompetent pig models. Under general anesthesia, 10 full-thickness skin defects (5 x 5 cm) were surgically created on the dorsal surface of each pig after antiseptic preparation. Fascial incisions were made in the defects to induce complicated deep wound and soft tissue infection (fasciitis and myonecrosis) and to maintain the infection for the duration of the experiment. For wound infection, a bacterial inoculum of MRSA NRL/Atb 5922 strain (sequence type ST22) was diffusely applied to sterile gauze covering the skin defects in 10 pigs or the multidrug-resistant clinical strain P. aeruginosa FF2 (VIM-carbapenemase-producing) in 6 pigs. Subsequently, all wounds of each pig were covered with an occlusive dressing and sterile gauze, and finally the pigs were dressed in an elastic bandage. Analgesia was achieved with the use of meloxicam for three consecutive days. Preoperative antibiotic prophylaxis was used to reduce the risk of contaminating bacterial infections affecting the induced infections. On days 4, 7, 10 and 14, wounds were visually assessed and biopsies were taken for microbiological and histological examination as well as for gene expression. There was no significant deterioration in the overall health of the animals during the experimental period. The cSSTI infection model for both pathogens was effectively established and maintained for 14 days. Due to the immunocompetence of the model animals, all immunological responses to the infectious agent and wound healing processes are preserved.Acknowledgements: The work was supported by Ministry of Health of the Czech Republic, Grant Numbers NV19-05-00214, NU20-05-00166 and NU22-05-00475.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů