Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F25%3A00144756" target="_blank" >RIV/00216224:14110/25:00144756 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.femsmicro.org/abstracts-overview" target="_blank" >https://www.femsmicro.org/abstracts-overview</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa

Popis výsledku v původním jazyce

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a significant threat to patients, often resulting in life-threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for CRPA complicated skin and soft tissue infections (cSSTI). The study presents a novel porcine model inducing and sustaining cSSTI caused by CRPA. Six fully immunocompetent pigs (120 wounds) were used to develop wound infection and evaluate the progression of cSSTI in the model. Full-thickness skin defects (5 x 5 cm) with fascial incisions were surgically performed to introduce cSSTI. CRPA clinical strain FF2 (producing VIM carbapenemase) was used for wound infection development. Microbiological and histopathological assessment was performed by processing the tissue biopsy samples. The model demonstrated bacterial loads of 107 CFU/gram of tissue and higher with extensive areas of bacterial cells within the wound tissue and on its surface. The cSSTI fully developed within three days and remained as such for 14 days post-infection. The histopathological evaluation confirmed the full development of cSSTI. The samples were characterized by significant tissue infiltration by inflammatory cells, with a predominance of polymorphonuclear cells and macrophages. This fully immunocompetent model of cSSTI caused by CRPA will serve in the development of novel therapeutical strategies (including antimicrobial peptides and enzymes, phage therapy, etc.) and corresponding preclinical studies. All immunological processes associated with the wound healing process (especially the inflammatory and proliferative phases) are preserved.

Název v anglickém jazyce

Fully Immunocompetent Porcine Model of Complicated Skin and Soft Tissue Infection Caused by Carbapenem-Resistant Pseudomonas aeruginosa

Popis výsledku anglicky

Carbapenem-resistant Pseudomonas aeruginosa (CRPA) poses a significant threat to patients, often resulting in life-threatening infections. With increasing antimicrobial resistance, novel therapeutic strategies are urgently needed. Although animal models are crucial for preclinical studies, limited data are available for porcine models, more specifically for CRPA complicated skin and soft tissue infections (cSSTI). The study presents a novel porcine model inducing and sustaining cSSTI caused by CRPA. Six fully immunocompetent pigs (120 wounds) were used to develop wound infection and evaluate the progression of cSSTI in the model. Full-thickness skin defects (5 x 5 cm) with fascial incisions were surgically performed to introduce cSSTI. CRPA clinical strain FF2 (producing VIM carbapenemase) was used for wound infection development. Microbiological and histopathological assessment was performed by processing the tissue biopsy samples. The model demonstrated bacterial loads of 107 CFU/gram of tissue and higher with extensive areas of bacterial cells within the wound tissue and on its surface. The cSSTI fully developed within three days and remained as such for 14 days post-infection. The histopathological evaluation confirmed the full development of cSSTI. The samples were characterized by significant tissue infiltration by inflammatory cells, with a predominance of polymorphonuclear cells and macrophages. This fully immunocompetent model of cSSTI caused by CRPA will serve in the development of novel therapeutical strategies (including antimicrobial peptides and enzymes, phage therapy, etc.) and corresponding preclinical studies. All immunological processes associated with the wound healing process (especially the inflammatory and proliferative phases) are preserved.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NU22-05-00475" target="_blank" >NU22-05-00475: Léčba infekcí způsobených multirezistentními kmeny bakterií pomocí nových antibakteriálních přípravků založených na katelicidinových antimikrobiálních peptidech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů