The role of extracellular vesicles in cell-to-cell communication

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000201" target="_blank" >RIV/00027162:_____/25:N0000201 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The role of extracellular vesicles in cell-to-cell communication

Popis výsledku v původním jazyce

Czech Annual Cancer Research Meeting 2025, ISBN 978-80-908348-4-2, Olomouc, 24.-26.11.2025, poster. Extracellular vesicles (EVs) are small particles surrounded by a lipid bilayer that cannot replicate on their own. They are released from cells into the external environment and are typically about 100 nm in size. Recent research shows that EVs are secreted from most cell types. In vitro cell cultures release EVs and other factors into the culture medium, creating what is known as conditioned cell culture medium. In the first part of our study, we compared the expression of specific cancer markers—such as EGFR, PD-L1, c-MET, and E-cadherin—between cell lines and the EVs derived from their conditioned medium. In the second part, we examined paired patient tissue samples (tumor and non-tumor) and the EVs isolated from them. EVs were extracted from the conditioned medium through ultracentrifugation, and the presence of these vesicles was confirmed using the CD63 marker. Subsequently, we measured the size, concentration and polydispersity index (PI) of the isolated EVs using multi-angle dynamic light scattering (MADLS). The characterization of EVs was further analyzed using flow cytometry analysis of small particles. Our results show that EGFR expression is consistent in samples from cell lines and EVs isolated from conditioned media. Both analyses indicate that EGFR levels are higher in tumor cell lines but lower in the non-tumor cell line, supporting the idea that EVs mirror the properties of the cells they come from. Elevated EGFR expression may suggest a tumor, but for accurate results, mutational analysis should be conducted to identify excessive or ongoing pathway stimulation. The expression levels of the CDH1 gene, which encodes E-cadherin, were analyzed in tissue patient samples using RT-qPCR. Notably, higher CDH1 expression was observed in tumor samples compared to non-tumor samples. Similarly, tissue EVs isolated from tumor samples showed higher E-cadherin expression compared to EVs from non-tumor samples. Our results showed that EVs isolated from conditioned media and tissue samples can contribute to intercellular communication, with the expression of examined proteins matching that of the originating cell sources.

Název v anglickém jazyce

The role of extracellular vesicles in cell-to-cell communication

Popis výsledku anglicky

Czech Annual Cancer Research Meeting 2025, ISBN 978-80-908348-4-2, Olomouc, 24.-26.11.2025, poster. Extracellular vesicles (EVs) are small particles surrounded by a lipid bilayer that cannot replicate on their own. They are released from cells into the external environment and are typically about 100 nm in size. Recent research shows that EVs are secreted from most cell types. In vitro cell cultures release EVs and other factors into the culture medium, creating what is known as conditioned cell culture medium. In the first part of our study, we compared the expression of specific cancer markers—such as EGFR, PD-L1, c-MET, and E-cadherin—between cell lines and the EVs derived from their conditioned medium. In the second part, we examined paired patient tissue samples (tumor and non-tumor) and the EVs isolated from them. EVs were extracted from the conditioned medium through ultracentrifugation, and the presence of these vesicles was confirmed using the CD63 marker. Subsequently, we measured the size, concentration and polydispersity index (PI) of the isolated EVs using multi-angle dynamic light scattering (MADLS). The characterization of EVs was further analyzed using flow cytometry analysis of small particles. Our results show that EGFR expression is consistent in samples from cell lines and EVs isolated from conditioned media. Both analyses indicate that EGFR levels are higher in tumor cell lines but lower in the non-tumor cell line, supporting the idea that EVs mirror the properties of the cells they come from. Elevated EGFR expression may suggest a tumor, but for accurate results, mutational analysis should be conducted to identify excessive or ongoing pathway stimulation. The expression levels of the CDH1 gene, which encodes E-cadherin, were analyzed in tissue patient samples using RT-qPCR. Notably, higher CDH1 expression was observed in tumor samples compared to non-tumor samples. Similarly, tissue EVs isolated from tumor samples showed higher E-cadherin expression compared to EVs from non-tumor samples. Our results showed that EVs isolated from conditioned media and tissue samples can contribute to intercellular communication, with the expression of examined proteins matching that of the originating cell sources.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30203 - Respiratory systems

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů