Expression of Cancer Markers in Extracellular Vesicles: Comparison Between Cell Lines, Conditioned Media, and Patient Tissue Samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F25%3AN0000203" target="_blank" >RIV/00027162:_____/25:N0000203 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Expression of Cancer Markers in Extracellular Vesicles: Comparison Between Cell Lines, Conditioned Media, and Patient Tissue Samples

Popis výsledku v původním jazyce

CzeSEV meeting on Extracellular Vesicles 2025 (ISBN of Proceedings: 978-80-11-07777-8), Vestec, 11.11.2025, poster. Precise and dynamic cell–cell communication is fundamental for tissue homeostasis, development, immune surveillance, and disease progression. In addition to direct contact, cytokines and growth factors, along with extracellular vesicles (EVs), are also released from cells into the surrounding environment. In this study, we investigated whether EVs reflect the molecular profile of the original tumor cells. EVs were isolated by ultracentrifugation and characterized using MADLS and flow cytometry of small particles. Initially, we examined the expression of key oncogenic markers such as EGFR, PD-L1, c-MET, and E-cadherin in human cancer cell lines. EGFR expression levels were consistently elevated in both tumor cell lines and their corresponding EVs. These findings support the hypothesis that EVs faithfully recapitulate the protein expression patterns of their parental cells. Next, RT-qPCR analysis of EGFR, PD-L1, c-MET, and E-cadherin was performed in tissue samples from both non-tumor and tumor areas. Moreover, we compared the expression of these tumor markers on EVs isolated from the conditioned media of paired tumor and non-tumor tissue samples. Notably, E-cadherin, a marker associated with epithelial phenotype, was upregulated in tumor tissue samples, even in EVs. Our data demonstrate that EVs derived from both conditioned media and human tissue carry diagnostically relevant molecular information about their origin. These findings underscore the potential utility of EVs as minimally invasive biomarkers for tumor characterization and diagnostics.

Název v anglickém jazyce

Expression of Cancer Markers in Extracellular Vesicles: Comparison Between Cell Lines, Conditioned Media, and Patient Tissue Samples

Popis výsledku anglicky

CzeSEV meeting on Extracellular Vesicles 2025 (ISBN of Proceedings: 978-80-11-07777-8), Vestec, 11.11.2025, poster. Precise and dynamic cell–cell communication is fundamental for tissue homeostasis, development, immune surveillance, and disease progression. In addition to direct contact, cytokines and growth factors, along with extracellular vesicles (EVs), are also released from cells into the surrounding environment. In this study, we investigated whether EVs reflect the molecular profile of the original tumor cells. EVs were isolated by ultracentrifugation and characterized using MADLS and flow cytometry of small particles. Initially, we examined the expression of key oncogenic markers such as EGFR, PD-L1, c-MET, and E-cadherin in human cancer cell lines. EGFR expression levels were consistently elevated in both tumor cell lines and their corresponding EVs. These findings support the hypothesis that EVs faithfully recapitulate the protein expression patterns of their parental cells. Next, RT-qPCR analysis of EGFR, PD-L1, c-MET, and E-cadherin was performed in tissue samples from both non-tumor and tumor areas. Moreover, we compared the expression of these tumor markers on EVs isolated from the conditioned media of paired tumor and non-tumor tissue samples. Notably, E-cadherin, a marker associated with epithelial phenotype, was upregulated in tumor tissue samples, even in EVs. Our data demonstrate that EVs derived from both conditioned media and human tissue carry diagnostically relevant molecular information about their origin. These findings underscore the potential utility of EVs as minimally invasive biomarkers for tumor characterization and diagnostics.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30203 - Respiratory systems

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů