Determination of retinoic acid in human serum and plasma by high-performance liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064165%3A_____%2F19%3A10409783" target="_blank" >RIV/00064165:_____/19:10409783 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10409783

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=LvFF_nJ--Y" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=LvFF_nJ--Y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-019-02489-1" target="_blank" >10.1007/s00706-019-02489-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of retinoic acid in human serum and plasma by high-performance liquid chromatography

Popis výsledku v původním jazyce

All-trans retinoic acid (ATRA) is the main derivative of retinol. It is formed in the intestinal mucosa from retinaldehyde. The aim was to establish a high-performance liquid chromatography method for determination of ATRA in human serum and plasma. Stability of ATRA in serum stored under various conditions was tested as well. ATRA was extracted from serum (plasma) after deproteination with acetonitrile by hexan:ethylacetate mixture (50:50). The extract was then evaporated under nitrogen at room temperature and dissolved in methanol. ATRA was detected after separation on C18 column at 355 nm. This method uses acitretin as internal standard. Limit of detection was 0.21 µg/dm3 and limit of quantification 0.70 µg/dm3. The method is linear for ATRA concentration 0.78-25 µg/dm3. Based on our results, ATRA has the same concentration in serum and in plasma. Repeatability of the method measured within run is given by variation coefficient 4.31% for serum and 1.86% for plasma. Reproducibility of the method measured between runs is given by variation coefficient 9.55% for serum and 8.00% for plasma. The worst storage conditions were light and room temperature, ATRA decreased by 0.69 µg/dm3, i.e., by 39%. Simultaneously, with ATRA, retinol is measured as well. We have introduced a fast, simple, and reliable method for determination of ATRA in human serum and plasma.

Název v anglickém jazyce

Determination of retinoic acid in human serum and plasma by high-performance liquid chromatography

Popis výsledku anglicky

All-trans retinoic acid (ATRA) is the main derivative of retinol. It is formed in the intestinal mucosa from retinaldehyde. The aim was to establish a high-performance liquid chromatography method for determination of ATRA in human serum and plasma. Stability of ATRA in serum stored under various conditions was tested as well. ATRA was extracted from serum (plasma) after deproteination with acetonitrile by hexan:ethylacetate mixture (50:50). The extract was then evaporated under nitrogen at room temperature and dissolved in methanol. ATRA was detected after separation on C18 column at 355 nm. This method uses acitretin as internal standard. Limit of detection was 0.21 µg/dm3 and limit of quantification 0.70 µg/dm3. The method is linear for ATRA concentration 0.78-25 µg/dm3. Based on our results, ATRA has the same concentration in serum and in plasma. Repeatability of the method measured within run is given by variation coefficient 4.31% for serum and 1.86% for plasma. Reproducibility of the method measured between runs is given by variation coefficient 9.55% for serum and 8.00% for plasma. The worst storage conditions were light and room temperature, ATRA decreased by 0.69 µg/dm3, i.e., by 39%. Simultaneously, with ATRA, retinol is measured as well. We have introduced a fast, simple, and reliable method for determination of ATRA in human serum and plasma.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Svazek periodika

150

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

5

Strana od-do

1731-1735

Kód UT WoS článku

000514591400001

EID výsledku v databázi Scopus

2-s2.0-85074212172