Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39898252" target="_blank" >RIV/00216275:25310/14:39898252 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1111/and.12103" target="_blank" >http://dx.doi.org/10.1111/and.12103</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/and.12103" target="_blank" >10.1111/and.12103</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection
Popis výsledku v původním jazyce
Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance
Název v anglickém jazyce
Determination of retinol and alpha-tocopherol in human seminal plasma using an HPLC with UV detection
Popis výsledku anglicky
Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and alpha-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and alpha-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and alpha-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for alpha-tocopherol. No significant differences in both retinol and alpha-tocopherol concentration between the smokers and nonsmokers (15 +/- 7nm and 1.86 +/- 0.29 mu m versus 15 +/- 6nm and 1.93 +/- 0.45 mu m) were found. A selective high-performance
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Andrologia
ISSN
0303-4569
e-ISSN
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Svazek periodika
46
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
DE - Spolková republika Německo
Počet stran výsledku
7
Strana od-do
472-478
Kód UT WoS článku
000335009600003
EID výsledku v databázi Scopus
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