Determination of Retinol, alpha-Tocopherol, Lycopene, and beta-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896326" target="_blank" >RIV/00216275:25310/13:39896326 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.hindawi.com/journals/jchem/2013/460242/" target="_blank" >http://www.hindawi.com/journals/jchem/2013/460242/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2013/460242" target="_blank" >10.1155/2013/460242</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of Retinol, alpha-Tocopherol, Lycopene, and beta-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
Popis výsledku v původním jazyce
A method is described here for the simultaneous determination of retinol, alpha-tocopherol, lycopene, and beta-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directed into the HPLC system. The separation was realized on analytical reversed-phase column with a UV-Vis detection. The analytical performance of this method was satisfactory. The intraassay and interaassay coefficientsof variation were below 10%. The recoveries were as follows. 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for alpha-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for beta-carotene. The levels of selected fat-soluble vitamins in plasma ofpatients with cardiovascular disease were measured and discussed.
Název v anglickém jazyce
Determination of Retinol, alpha-Tocopherol, Lycopene, and beta-Carotene in Human Plasma Using HPLC with UV-Vis Detection: Application to a Clinical Study
Popis výsledku anglicky
A method is described here for the simultaneous determination of retinol, alpha-tocopherol, lycopene, and beta-carotene in human plasma. The effectiveness of various protein precipitants and extraction solvents was tested. After adequate sample preparation, the samples were injected directed into the HPLC system. The separation was realized on analytical reversed-phase column with a UV-Vis detection. The analytical performance of this method was satisfactory. The intraassay and interaassay coefficientsof variation were below 10%. The recoveries were as follows. 97.0% (CV 2.4%) for retinol, 94.6% (CV 1.7%) for alpha-tocopherol, 91.9% (CV 3.6%) for lycopene, and 93.9% (CV 4.2%) for beta-carotene. The levels of selected fat-soluble vitamins in plasma ofpatients with cardiovascular disease were measured and discussed.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Chemistry
ISSN
2090-9063
e-ISSN
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Svazek periodika
2013
Číslo periodika v rámci svazku
2013
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
"460242-1"-"460242-7"
Kód UT WoS článku
000311147500001
EID výsledku v databázi Scopus
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