The effect of selected pre-analytical phase variables on plasma thromboxane A2 measurements in humans

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F13%3A%230000350" target="_blank" >RIV/00064173:_____/13:#0000350 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1751-553X.2012.01458.x" target="_blank" >http://dx.doi.org/10.1111/j.1751-553X.2012.01458.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1751-553X.2012.01458.x" target="_blank" >10.1111/j.1751-553X.2012.01458.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of selected pre-analytical phase variables on plasma thromboxane A2 measurements in humans

Popis výsledku v původním jazyce

Aims: Platelet function testing is often affected by the existence of different pre-analytical variables that can cause platelet activation. The aim of this study was to assess the effect of such variables that are present when samples are taken (different anticoagulants, incubation temperature, and storage conditions) to select those which enable to reach optimal range of measured plasma concentrations of the two stable thromboxane A2 metabolites, that is, thromboxane B2 (TxB2) and 11-dehydrothromboxane B2 (11-dTxB2). Materials and Methods: For the purpose of this study, whole blood samples obtained from 20 volunteers were screened for TxB2 and 11-dTxB2 concentrations using commercial EIA kits (Cayman Chemicals; Neogen) in relation to the effect of different anticoagulants, using different incubation temperatures and storage conditions. Results:Trisodium citrate has been shown not to be affecting the TxB2 and 11-dTxB2 concentrations compared with the values measured in the serum. Incu

Název v anglickém jazyce

The effect of selected pre-analytical phase variables on plasma thromboxane A2 measurements in humans

Popis výsledku anglicky

Aims: Platelet function testing is often affected by the existence of different pre-analytical variables that can cause platelet activation. The aim of this study was to assess the effect of such variables that are present when samples are taken (different anticoagulants, incubation temperature, and storage conditions) to select those which enable to reach optimal range of measured plasma concentrations of the two stable thromboxane A2 metabolites, that is, thromboxane B2 (TxB2) and 11-dehydrothromboxane B2 (11-dTxB2). Materials and Methods: For the purpose of this study, whole blood samples obtained from 20 volunteers were screened for TxB2 and 11-dTxB2 concentrations using commercial EIA kits (Cayman Chemicals; Neogen) in relation to the effect of different anticoagulants, using different incubation temperatures and storage conditions. Results:Trisodium citrate has been shown not to be affecting the TxB2 and 11-dTxB2 concentrations compared with the values measured in the serum. Incu

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scandinavian Journal of Clinical & Laboratory Investigation

ISSN

0036-5513

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

92-100

Kód UT WoS článku

000316146100018

EID výsledku v databázi Scopus

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