The effect of selected pre-analytical phase variables on plasma thromboxane A(2) measurements in humans

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064190%3A_____%2F13%3A%230000472" target="_blank" >RIV/00064190:_____/13:#0000472 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/13:43905462

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1751-553X.2012.01458.x" target="_blank" >http://dx.doi.org/10.1111/j.1751-553X.2012.01458.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1751-553X.2012.01458.x" target="_blank" >10.1111/j.1751-553X.2012.01458.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of selected pre-analytical phase variables on plasma thromboxane A(2) measurements in humans

Popis výsledku v původním jazyce

Aims: Platelet function testing is often affected by the existence of different pre-analytical variables that can cause platelet activation. The aim of this study was to assess the effect of such variables that are present when samples are taken (different anticoagulants, incubation temperature, and storage conditions) to select those which enable to reach optimal range of measured plasma concentrations of the two stable thromboxane A(2) metabolites, that is, thromboxane B-2 (TxB(2)) and 11-dehydrothromboxane B-2 (11-dTxB(2)). Materials and Methods: For the purpose of this study, whole blood samples obtained from 20 volunteers were screened for TxB(2) and 11-dTxB(2) concentrations using commercial EIA kits (Cayman ChemicalsTM; NeogenTM) in relation tothe effect of different anticoagulants, using different incubation temperatures and storage conditions. Results: Trisodium citrate has been shown not to be affecting the TxB(2) and 11-dTxB(2) concentrations compared with the values measur

Název v anglickém jazyce

The effect of selected pre-analytical phase variables on plasma thromboxane A(2) measurements in humans

Popis výsledku anglicky

Aims: Platelet function testing is often affected by the existence of different pre-analytical variables that can cause platelet activation. The aim of this study was to assess the effect of such variables that are present when samples are taken (different anticoagulants, incubation temperature, and storage conditions) to select those which enable to reach optimal range of measured plasma concentrations of the two stable thromboxane A(2) metabolites, that is, thromboxane B-2 (TxB(2)) and 11-dehydrothromboxane B-2 (11-dTxB(2)). Materials and Methods: For the purpose of this study, whole blood samples obtained from 20 volunteers were screened for TxB(2) and 11-dTxB(2) concentrations using commercial EIA kits (Cayman ChemicalsTM; NeogenTM) in relation tothe effect of different anticoagulants, using different incubation temperatures and storage conditions. Results: Trisodium citrate has been shown not to be affecting the TxB(2) and 11-dTxB(2) concentrations compared with the values measur

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY

ISSN

1751-5521

e-ISSN

—

Svazek periodika

35

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

92-100

Kód UT WoS článku

000316146100018

EID výsledku v databázi Scopus

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