Significant hemolysis is present during irreversible electroporation of cardiomyocytes in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064173%3A_____%2F25%3A43927461" target="_blank" >RIV/00064173:_____/25:43927461 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68407700:21460/25:00381377 RIV/00216224:14110/25:00142557 RIV/00216208:11120/25:43927461

Výsledek na webu

<a href="https://doi.org/10.1016/j.hrthm.2024.08.019" target="_blank" >https://doi.org/10.1016/j.hrthm.2024.08.019</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.hrthm.2024.08.019" target="_blank" >10.1016/j.hrthm.2024.08.019</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Significant hemolysis is present during irreversible electroporation of cardiomyocytes in vitro

Popis výsledku v původním jazyce

BACKGROUND: Pulsed-field ablation (PFA) of atrial fibrillation (AF) is a new method in clinical practice. Despite a favorable safety profile of PFA in AF ablation, rare cases of renal failure, probably due to hemolysis, have been recently reported. OBJECTIVE: The aim of this study was to determine the rate of hemolysis and cardiac cell death during in vitro PFA with different electric field intensities. METHODS: Blood samples from healthy volunteers and mouse HL-1 cardiomyocyte cell lines were subjected to in vitro irreversible electroporation (IRE) using 216 bipolar pulses, each lasting 2 μs with 5 μs intervals, repeated 20 times at a frequency of 1 Hz. These pulses varied in from 500 to 1500 V. Cell-free hemoglobin levels were assessed spectrophotometrically, and red blood cell microparticles (RBCμ) were evaluated using flow cytometry. Cardiomyocyte death was quantified using propidium iodide. RESULTS: PF energy (1000 V/cm, 1250 V/cm, and 1500 V/cm) was associated with a significant increase in cell-free hemoglobin (0.31 +- 0.16 g/l, 2.33 +- 0.90 g/l, and 5.7 +- 0.20 g/l, p&lt; 0.05), and similar increase in the concentration of RBCμ. Significant rates of cardiomyocyte death were observed at electric field strengths of 750 V/cm, 1000 V/cm, 1250 V/cm and 1500 V/cm (26.5 +- 5.9%, 44.3 +- 6.2%, 55.5 +- 6.9% and 74.5 +- 17.8% of cardiomyocytes, p &lt; 0.05). CONCLUSION: The most effective induction of cell death in vitro was observed at 1500 V/cm. This intensity was also associated with a significant degree of hemolysis.

Název v anglickém jazyce

Significant hemolysis is present during irreversible electroporation of cardiomyocytes in vitro

Popis výsledku anglicky

BACKGROUND: Pulsed-field ablation (PFA) of atrial fibrillation (AF) is a new method in clinical practice. Despite a favorable safety profile of PFA in AF ablation, rare cases of renal failure, probably due to hemolysis, have been recently reported. OBJECTIVE: The aim of this study was to determine the rate of hemolysis and cardiac cell death during in vitro PFA with different electric field intensities. METHODS: Blood samples from healthy volunteers and mouse HL-1 cardiomyocyte cell lines were subjected to in vitro irreversible electroporation (IRE) using 216 bipolar pulses, each lasting 2 μs with 5 μs intervals, repeated 20 times at a frequency of 1 Hz. These pulses varied in from 500 to 1500 V. Cell-free hemoglobin levels were assessed spectrophotometrically, and red blood cell microparticles (RBCμ) were evaluated using flow cytometry. Cardiomyocyte death was quantified using propidium iodide. RESULTS: PF energy (1000 V/cm, 1250 V/cm, and 1500 V/cm) was associated with a significant increase in cell-free hemoglobin (0.31 +- 0.16 g/l, 2.33 +- 0.90 g/l, and 5.7 +- 0.20 g/l, p&lt; 0.05), and similar increase in the concentration of RBCμ. Significant rates of cardiomyocyte death were observed at electric field strengths of 750 V/cm, 1000 V/cm, 1250 V/cm and 1500 V/cm (26.5 +- 5.9%, 44.3 +- 6.2%, 55.5 +- 6.9% and 74.5 +- 17.8% of cardiomyocytes, p &lt; 0.05). CONCLUSION: The most effective induction of cell death in vitro was observed at 1500 V/cm. This intensity was also associated with a significant degree of hemolysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30201 - Cardiac and Cardiovascular systems

Projekt

<a href="/cs/project/LX22NPO5104" target="_blank" >LX22NPO5104: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Heart Rhythm

ISSN

1547-5271

e-ISSN

1556-3871

Svazek periodika

22

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

466-474

Kód UT WoS článku

001423173300001

EID výsledku v databázi Scopus

2-s2.0-85203423403