Expulsion of amplified MYCN from homogenously staining chromosomal regions in neuroblastoma cell lines after cultivation with cisplatin, doxorubicin, hydroxyurea, and vincristine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6219" target="_blank" >RIV/00064203:_____/10:6219 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/10:6219

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Expulsion of amplified MYCN from homogenously staining chromosomal regions in neuroblastoma cell lines after cultivation with cisplatin, doxorubicin, hydroxyurea, and vincristine

Popis výsledku v původním jazyce

Amplified MYCN, common in neuroblastomas, can be detected as double minutes (dmin) or homogenously staining chromosomal regions (hsr). Expulsion of amplified MYCN has only been described in dmin. We used hydroxyurea, which accelerates the expulsion of amplified genes and cytostatics used in neuroblastoma therapy, to describe MYCN amplification changes after chemotherapy. The loss of amplified MYCN copies was investigated using CGH and FISH. We found expulsion of amplified MYCN from hsr and determined the exact number of amplified MYCN copies. Our data show that expulsion of amplified MYCN genes is also possible from hsr and may be induced, not only by HU, but by other cytostatics as well.

Název v anglickém jazyce

Expulsion of amplified MYCN from homogenously staining chromosomal regions in neuroblastoma cell lines after cultivation with cisplatin, doxorubicin, hydroxyurea, and vincristine

Popis výsledku anglicky

Amplified MYCN, common in neuroblastomas, can be detected as double minutes (dmin) or homogenously staining chromosomal regions (hsr). Expulsion of amplified MYCN has only been described in dmin. We used hydroxyurea, which accelerates the expulsion of amplified genes and cytostatics used in neuroblastoma therapy, to describe MYCN amplification changes after chemotherapy. The loss of amplified MYCN copies was investigated using CGH and FISH. We found expulsion of amplified MYCN from hsr and determined the exact number of amplified MYCN copies. Our data show that expulsion of amplified MYCN genes is also possible from hsr and may be induced, not only by HU, but by other cytostatics as well.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cancer Genetics and Cytogenetics

ISSN

0165-4608

e-ISSN

—

Svazek periodika

196

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

000277395600015

EID výsledku v databázi Scopus

—