In vitro and in vivo effects of reovirus on HPV16-transformed mice cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6230" target="_blank" >RIV/00064203:_____/10:6230 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/10:6230 RIV/00023736:_____/10:00008655

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro and in vivo effects of reovirus on HPV16-transformed mice cells

Popis výsledku v původním jazyce

Oncolytic viruses are examined as anticancer therapeutics. In presented experiments we have employed two HPV16-transformed mouse cell lines, TC-1 and MK16, and reovirus type 3, strain Dearing (RV). Both lines are susceptible to RV and produce infectiousvirus in vitro while normal human cells are not susceptible to RV. TC-1 cells produced lesser amounts of virus, but died more rapidly than MK16. When inoculating high doses of infected cells into syngeneic animals their oncogenic activity was suppressedin the case of MK16 cells and less efficiently in the case of TC-1. Immunizing experiments in which non-oncogenic doses of RV infected TC-1 cells were compared with irradiated cells brought surprising results. When immunized animals were challenged withTC-1 cells, the irradiated cells proved to be a better immunogen that the infected ones. But, when challenged with MK16 cells the opposite was true. This difference was associated with the different properties of the cell lines tested.

Název v anglickém jazyce

In vitro and in vivo effects of reovirus on HPV16-transformed mice cells

Popis výsledku anglicky

Oncolytic viruses are examined as anticancer therapeutics. In presented experiments we have employed two HPV16-transformed mouse cell lines, TC-1 and MK16, and reovirus type 3, strain Dearing (RV). Both lines are susceptible to RV and produce infectiousvirus in vitro while normal human cells are not susceptible to RV. TC-1 cells produced lesser amounts of virus, but died more rapidly than MK16. When inoculating high doses of infected cells into syngeneic animals their oncogenic activity was suppressedin the case of MK16 cells and less efficiently in the case of TC-1. Immunizing experiments in which non-oncogenic doses of RV infected TC-1 cells were compared with irradiated cells brought surprising results. When immunized animals were challenged withTC-1 cells, the irradiated cells proved to be a better immunogen that the infected ones. But, when challenged with MK16 cells the opposite was true. This difference was associated with the different properties of the cell lines tested.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F05%2F2240" target="_blank" >GA301/05/2240: Imunitní mechanizmy v protinádorovém účinku onkolytického viru</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neoplasma

ISSN

0028-2685

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000277412800003

EID výsledku v databázi Scopus

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