A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6243" target="_blank" >RIV/00064203:_____/10:6243 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/10:6243

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma

Popis výsledku v původním jazyce

A clinical course of patients with mantle cell lymphoma (MCL) is aggressive, and the disease is rarely curable. Proliferation rate is the most important prognostic factor. We developed a novel, reliable, rapid, and routinely applicable approach allowinga precise quantitative assessment of three proliferation markers, Ki-67, topoisomerase II alpha, and TPX2. We employed real-time reverse transcription PCR (RQ-RT-PCR). We observed individual variability of the mRNA expression levels, reflecting heterogeneity of the proliferation rate in individual patients. In general, we observed the highest mRNA expression in the group of Burkitt lymphomas and the lowest in patients with reactive lymphadenopathies. We found increased proliferation rate in MCLs with high cyclin D1 mRNA indicating a quantitative cell cycle control. We observed a correlation between mRNA level and the immunohistochemical staining of corresponding proteins which significantly argues for the prognostic significance of the

Název v anglickém jazyce

A novel quantitative PCR of proliferation markers (Ki-67, topoisomerase II alpha, and TPX2): an immunohistochemical correlation, testing, and optimizing for mantle cell lymphoma

Popis výsledku anglicky

A clinical course of patients with mantle cell lymphoma (MCL) is aggressive, and the disease is rarely curable. Proliferation rate is the most important prognostic factor. We developed a novel, reliable, rapid, and routinely applicable approach allowinga precise quantitative assessment of three proliferation markers, Ki-67, topoisomerase II alpha, and TPX2. We employed real-time reverse transcription PCR (RQ-RT-PCR). We observed individual variability of the mRNA expression levels, reflecting heterogeneity of the proliferation rate in individual patients. In general, we observed the highest mRNA expression in the group of Burkitt lymphomas and the lowest in patients with reactive lymphadenopathies. We found increased proliferation rate in MCLs with high cyclin D1 mRNA indicating a quantitative cell cycle control. We observed a correlation between mRNA level and the immunohistochemical staining of corresponding proteins which significantly argues for the prognostic significance of the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Virchows Archiv

ISSN

0945-6317

e-ISSN

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Svazek periodika

456

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000278363800010

EID výsledku v databázi Scopus

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