Effects of redox conditions and zinc(II) ions on metallothionein aggregation revealed by chip capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F10%3A6387" target="_blank" >RIV/00064203:_____/10:6387 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/10:00040656 RIV/62156489:43210/10:00166516 RIV/00216208:11130/10:6387

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of redox conditions and zinc(II) ions on metallothionein aggregation revealed by chip capillary electrophoresis

Popis výsledku v původním jazyce

We used capillary electrophoresis on chip coupled with fluorescence detection to determine structural changes of metallothionein (MT) with increasing concentration of zinc(II) ions and under various redox conditions. To investigate the structural-dependent effects, reduced and oxidized apo-MT was prepared. Zinc binding into reduced and oxidized apo-MT was compared. Formation of MT aggregates with increasing zinc concentration was observed by spectrophotometry, chip capillary electrophoresis, and SDS-PAGE. We found out that reduced MT forms aggregates more readily compared to oxidized MT. Using the chip capillary electrophoresis allowed us quantification of MT aggregation as a decrease in the area of the signal corresponding to the monomer form of MT and its ratio to total signal. The dependences had an exponential character. Zn-MT interaction was 30% faster during the first 15 min and 50% faster during the whole experiment for reduced MT.

Název v anglickém jazyce

Effects of redox conditions and zinc(II) ions on metallothionein aggregation revealed by chip capillary electrophoresis

Popis výsledku anglicky

We used capillary electrophoresis on chip coupled with fluorescence detection to determine structural changes of metallothionein (MT) with increasing concentration of zinc(II) ions and under various redox conditions. To investigate the structural-dependent effects, reduced and oxidized apo-MT was prepared. Zinc binding into reduced and oxidized apo-MT was compared. Formation of MT aggregates with increasing zinc concentration was observed by spectrophotometry, chip capillary electrophoresis, and SDS-PAGE. We found out that reduced MT forms aggregates more readily compared to oxidized MT. Using the chip capillary electrophoresis allowed us quantification of MT aggregation as a decrease in the area of the signal corresponding to the monomer form of MT and its ratio to total signal. The dependences had an exponential character. Zn-MT interaction was 30% faster during the first 15 min and 50% faster during the whole experiment for reduced MT.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1217

Číslo periodika v rámci svazku

51

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

—

Kód UT WoS článku

000285429700006

EID výsledku v databázi Scopus

—