Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F10%3A00184955" target="_blank" >RIV/62156489:43210/10:00184955 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines

Popis výsledku v původním jazyce

Background: Metallothioneins (MTs) are heavy-metal binding cysteine-rich proteins. MTs are thought to play a role in tumour disease. They can be used for studying of course, prognosis and metastating of a tumour. The enhanced MTs expression leads to theformation of cytostatics resistance in tumour cells. One the current accepted opinion is that these mechanisms are closely connected to ability of MT to bind heavy metal ions and form aggregates. The aim of this work was to study the changes in aggregation of MT under various concentrations of zinc and different redox conditions in human prostatic cell lines. Material and Methods: Reduced apo-MT was oxidized with 0.75 and 3.5 % H2O2, 0.5 and 1 mMK2Cr2O7, and 0.5 and 1 mM KMnO4 for 2 hours at 37C. To thereduced and oxidized apo-MT ZnCl2 was added. Reaction was measured spectrometrically for 1 hour at 37 C. MT oxidation and interaction with zinc was evaluated by measuring of spectra within the range from 200 to 300 nm. Human cancer prost

Název v anglickém jazyce

Study of zinc-dependent aggregation of metallothionein from human prostatic cancer cell lines

Popis výsledku anglicky

Background: Metallothioneins (MTs) are heavy-metal binding cysteine-rich proteins. MTs are thought to play a role in tumour disease. They can be used for studying of course, prognosis and metastating of a tumour. The enhanced MTs expression leads to theformation of cytostatics resistance in tumour cells. One the current accepted opinion is that these mechanisms are closely connected to ability of MT to bind heavy metal ions and form aggregates. The aim of this work was to study the changes in aggregation of MT under various concentrations of zinc and different redox conditions in human prostatic cell lines. Material and Methods: Reduced apo-MT was oxidized with 0.75 and 3.5 % H2O2, 0.5 and 1 mMK2Cr2O7, and 0.5 and 1 mM KMnO4 for 2 hours at 37C. To thereduced and oxidized apo-MT ZnCl2 was added. Reaction was measured spectrometrically for 1 hour at 37 C. MT oxidation and interaction with zinc was evaluated by measuring of spectra within the range from 200 to 300 nm. Human cancer prost

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Cancer

ISSN

0959-8049

e-ISSN

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Svazek periodika

8

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

1

Strana od-do

49

Kód UT WoS článku

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EID výsledku v databázi Scopus

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