Development of High-Sensitive ELISA Method for Detection of Adipophilin Levels in Human Colostrum and Breast Milk
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F14%3A10292991" target="_blank" >RIV/00064203:_____/14:10292991 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11130/14:10292991 RIV/62157124:16370/14:43872867
Výsledek na webu
<a href="http://dx.doi.org/10.1002/jcla.21675" target="_blank" >http://dx.doi.org/10.1002/jcla.21675</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jcla.21675" target="_blank" >10.1002/jcla.21675</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Development of High-Sensitive ELISA Method for Detection of Adipophilin Levels in Human Colostrum and Breast Milk
Popis výsledku v původním jazyce
Aim: To develop and validate high-sensitive (hs) ELISA method for detection of adipophilin (adipose differentiation-related protein, ADRP) in human breast milk (BM) and to analyze adipophilin levels in BM during 12 months of lactation. Methods: ADRP levels were determined using hsELISA method (Biovendor-Laboratory Medicine, Inc.) in colostrum (D0) and BM of 72 mothers was collected 1, 3, 6, and 12 months following delivery (M1, 3, 6, 12). Results: ADRP was detectable in BM up to 12 months of lactation.Mean levels at D0 were 1.98 +/- 0.12; M1, 2.83 +/- 0.21; M3, 2.39 +/- 0.17; M6, 2.57 +/- 0.16; and at M12 3.25 +/- 0.21 g/ml. Significantly higher levels of ADRP were found in M1 and M12 when compared to D0 and in M12 when compared to M3 (overall P = 0.0001). No significant correlation was seen between ADRP levels in BM and adiponectin, body weight of infants, their birth length, body weight gain during the first year of life, or BMI of mothers before pregnancy. Conclusions: We developed
Název v anglickém jazyce
Development of High-Sensitive ELISA Method for Detection of Adipophilin Levels in Human Colostrum and Breast Milk
Popis výsledku anglicky
Aim: To develop and validate high-sensitive (hs) ELISA method for detection of adipophilin (adipose differentiation-related protein, ADRP) in human breast milk (BM) and to analyze adipophilin levels in BM during 12 months of lactation. Methods: ADRP levels were determined using hsELISA method (Biovendor-Laboratory Medicine, Inc.) in colostrum (D0) and BM of 72 mothers was collected 1, 3, 6, and 12 months following delivery (M1, 3, 6, 12). Results: ADRP was detectable in BM up to 12 months of lactation.Mean levels at D0 were 1.98 +/- 0.12; M1, 2.83 +/- 0.21; M3, 2.39 +/- 0.17; M6, 2.57 +/- 0.16; and at M12 3.25 +/- 0.21 g/ml. Significantly higher levels of ADRP were found in M1 and M12 when compared to D0 and in M12 when compared to M3 (overall P = 0.0001). No significant correlation was seen between ADRP levels in BM and adiponectin, body weight of infants, their birth length, body weight gain during the first year of life, or BMI of mothers before pregnancy. Conclusions: We developed
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FG - Pediatrie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Clinical Laboratory Analysis
ISSN
0887-8013
e-ISSN
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Svazek periodika
28
Číslo periodika v rámci svazku
4
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
6
Strana od-do
255-260
Kód UT WoS článku
000339551900001
EID výsledku v databázi Scopus
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