Homeobox gene expression in acute myeloid leukemia is linked to typical underlying molecular aberrations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F14%3A10293647" target="_blank" >RIV/00064203:_____/14:10293647 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/14:10293647

Výsledek na webu

<a href="http://dx.doi.org/10.1186/s13045-014-0094-0" target="_blank" >http://dx.doi.org/10.1186/s13045-014-0094-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13045-014-0094-0" target="_blank" >10.1186/s13045-014-0094-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Homeobox gene expression in acute myeloid leukemia is linked to typical underlying molecular aberrations

Popis výsledku v původním jazyce

Background: Although distinct patterns of homeobox (HOX) gene expression have been described in defined cytogenetic and molecular subsets of patients with acute myeloid leukemia (AML), it is unknown whether these patterns are the direct result of transcriptional alterations or rather represent the differentiation stage of the leukemic cell. Method: To address this question, we used qPCR to analyze mRNA expression of HOXA and HOXB genes in bone marrow (BM) samples of 46 patients with AML and sorted subpopulations of healthy BM cells. These various stages of myeloid differentiation represent matched counterparts of morphological subgroups of AML. To further study the transcriptional alterations of HOX genes in hematopoiesis, we also analyzed gene expression of epigenetic modifiers in the subpopluations of healthy BM and leukemic cells. Results: Unsupervised hierarchical clustering divided the AMLs into five clusters characterized by the presence of prevalent molecular genetic aberrations

Název v anglickém jazyce

Homeobox gene expression in acute myeloid leukemia is linked to typical underlying molecular aberrations

Popis výsledku anglicky

Background: Although distinct patterns of homeobox (HOX) gene expression have been described in defined cytogenetic and molecular subsets of patients with acute myeloid leukemia (AML), it is unknown whether these patterns are the direct result of transcriptional alterations or rather represent the differentiation stage of the leukemic cell. Method: To address this question, we used qPCR to analyze mRNA expression of HOXA and HOXB genes in bone marrow (BM) samples of 46 patients with AML and sorted subpopulations of healthy BM cells. These various stages of myeloid differentiation represent matched counterparts of morphological subgroups of AML. To further study the transcriptional alterations of HOX genes in hematopoiesis, we also analyzed gene expression of epigenetic modifiers in the subpopluations of healthy BM and leukemic cells. Results: Unsupervised hierarchical clustering divided the AMLs into five clusters characterized by the presence of prevalent molecular genetic aberrations

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP304%2F12%2F2214" target="_blank" >GAP304/12/2214: Transkripční regulace HOX genů v normální a leukemické krvetvorbě</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Hematology and Oncology

ISSN

1756-8722

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

neuveden

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

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Kód UT WoS článku

000350000600001

EID výsledku v databázi Scopus

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