Vancomycin and Chitosan Modified Magnetic Particles for Antibacterial Prophylaxis in Orthopaedics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F24%3A10479854" target="_blank" >RIV/00064203:_____/24:10479854 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/24:10479854

Výsledek na webu

<a href="https://doi.org/10.37904/nanocon.2023.4794" target="_blank" >https://doi.org/10.37904/nanocon.2023.4794</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2023.4794" target="_blank" >10.37904/nanocon.2023.4794</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Vancomycin and Chitosan Modified Magnetic Particles for Antibacterial Prophylaxis in Orthopaedics

Popis výsledku v původním jazyce

Multiresistant bacterial strains are a very serious clinical problem in a hospital environment. Solving these types of nosocomial infections requires new and unique approaches. It is known that vancomycin is an antibiotic used for the treatment of serious infections such as S. aureus strains. Nanomedicine using new nanotechnologies brings new options like application of surface coating modifications for the targeting treatment. Superparamagnetic iron oxide nanoparticles (SPION) particles were created by a physicochemical method of synthesis. For the purpose of this testing, SPION particles have been modified by chitosan (300 rpm, 25 °C, 24 h), then purified on the magnet. SPION/Chito particles were modified by vancomycin (300 rpm, 25 °C, 6 h). The completed particles were washed with a PBS buffer (pH 7). The concentration of vancomycin (after the modifications) in SPION/Chito/VANCO particles was 100 µg/ml. The stability of finalized particles was tested in PBS (pH 7). The concentration of vancomycin was determined by HPLC. After that, the antibacterial activity was determined by measurement of growth curves (methicillin sensitive and resistant S. aureus) at 450 nm/540 nm for 24 hours (25 °C, 300 rpm in LB medium). The results were evaluated as AUC growth curves. Bacterial cultures were grown reproducibly in pure medium (AUCs were considered baseline). The study provided initial information about the effect of newly prepared types of nanoparticles on S. aureus species.

Název v anglickém jazyce

Vancomycin and Chitosan Modified Magnetic Particles for Antibacterial Prophylaxis in Orthopaedics

Popis výsledku anglicky

Multiresistant bacterial strains are a very serious clinical problem in a hospital environment. Solving these types of nosocomial infections requires new and unique approaches. It is known that vancomycin is an antibiotic used for the treatment of serious infections such as S. aureus strains. Nanomedicine using new nanotechnologies brings new options like application of surface coating modifications for the targeting treatment. Superparamagnetic iron oxide nanoparticles (SPION) particles were created by a physicochemical method of synthesis. For the purpose of this testing, SPION particles have been modified by chitosan (300 rpm, 25 °C, 24 h), then purified on the magnet. SPION/Chito particles were modified by vancomycin (300 rpm, 25 °C, 6 h). The completed particles were washed with a PBS buffer (pH 7). The concentration of vancomycin (after the modifications) in SPION/Chito/VANCO particles was 100 µg/ml. The stability of finalized particles was tested in PBS (pH 7). The concentration of vancomycin was determined by HPLC. After that, the antibacterial activity was determined by measurement of growth curves (methicillin sensitive and resistant S. aureus) at 450 nm/540 nm for 24 hours (25 °C, 300 rpm in LB medium). The results were evaluated as AUC growth curves. Bacterial cultures were grown reproducibly in pure medium (AUCs were considered baseline). The study provided initial information about the effect of newly prepared types of nanoparticles on S. aureus species.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30100 - Basic medicine

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings 15th International Conference on Nanomaterials - Research &amp; Application

ISBN

978-80-88365-15-0

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

6

Strana od-do

262-267

Název nakladatele

Tanger

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

18. 10. 2023

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

001234125400042