Separation and Analysis of Methicillin-sensitive and Resistant Staphylococcus Strains Using Vancomycin-modified Magnetic Nanoparticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064203%3A_____%2F25%3A10504269" target="_blank" >RIV/00064203:_____/25:10504269 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43510/25:43927557 RIV/00216208:11130/25:10504269

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=RBC81ah25Q" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=RBC81ah25Q</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/sscp.70135" target="_blank" >10.1002/sscp.70135</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Separation and Analysis of Methicillin-sensitive and Resistant Staphylococcus Strains Using Vancomycin-modified Magnetic Nanoparticles

Popis výsledku v původním jazyce

Multi-resistant bacterial strains pose a serious threat in the hospital environment. Management of nosocomial infections requires completely new approaches. Vancomycin (Vanco) is a known antibiotic for the treatment of serious infections, including those caused by methicillin-resistant Staphylococcus aureus strains. Nanomedicine brings new possibilities, including targeting using surface modifications of nanoparticles (NPs). Superparamagnetic iron oxide NPs (SPIONs) are synthetic nanomaterials with unique magnetic properties that are widely used in nanomedicine. They are primarily used in diagnostic imaging, targeted drug delivery, and therapy. Minimal inhibition concentration was determined to be 150 µg/mL. For the purpose of this study, SPIONs were modified with chitosan and then purified on a magnet. After washing with buffer, the particles were modified with Vanco (100 µg/mL). The stability of modified NPs was verified by Vanco analysis using high-performance liquid chromatography. Bacterial cultures were grown reproducibly in a pure medium. Subsequently, antibacterial activity was monitored by growth curves (S. aureus) that were measured at 450 nm/540 nm for 24 h, repeated in four cycles. The results were evaluated as the area under the growth curves. The study provides initial insights into the effects of newly synthesized NP variants on S. aureus.

Název v anglickém jazyce

Separation and Analysis of Methicillin-sensitive and Resistant Staphylococcus Strains Using Vancomycin-modified Magnetic Nanoparticles

Popis výsledku anglicky

Multi-resistant bacterial strains pose a serious threat in the hospital environment. Management of nosocomial infections requires completely new approaches. Vancomycin (Vanco) is a known antibiotic for the treatment of serious infections, including those caused by methicillin-resistant Staphylococcus aureus strains. Nanomedicine brings new possibilities, including targeting using surface modifications of nanoparticles (NPs). Superparamagnetic iron oxide NPs (SPIONs) are synthetic nanomaterials with unique magnetic properties that are widely used in nanomedicine. They are primarily used in diagnostic imaging, targeted drug delivery, and therapy. Minimal inhibition concentration was determined to be 150 µg/mL. For the purpose of this study, SPIONs were modified with chitosan and then purified on a magnet. After washing with buffer, the particles were modified with Vanco (100 µg/mL). The stability of modified NPs was verified by Vanco analysis using high-performance liquid chromatography. Bacterial cultures were grown reproducibly in a pure medium. Subsequently, antibacterial activity was monitored by growth curves (S. aureus) that were measured at 450 nm/540 nm for 24 h, repeated in four cycles. The results were evaluated as the area under the growth curves. The study provides initial insights into the effects of newly synthesized NP variants on S. aureus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30100 - Basic medicine

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Separation Science Plus

ISSN

2573-1815

e-ISSN

2573-1815

Svazek periodika

8

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

e70135

Kód UT WoS článku

001605964600005

EID výsledku v databázi Scopus

2-s2.0-105018604416