The role of alpha beta T-cells in spontaneous regression of melanoma tumors in swine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064211%3A_____%2F19%3AW0002028" target="_blank" >RIV/00064211:_____/19:W0002028 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/19:00503686 RIV/67985904:_____/19:00503686 RIV/60162694:G44__/19:00536529 RIV/60460709:41210/19:78673

Výsledek na webu

<a href="https://dx.doi.org/10.1016/j.dci.2018.10.001" target="_blank" >https://dx.doi.org/10.1016/j.dci.2018.10.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.dci.2018.10.001" target="_blank" >10.1016/j.dci.2018.10.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of alpha beta T-cells in spontaneous regression of melanoma tumors in swine

Popis výsledku v původním jazyce

Using a porcine model, we describe Melanoma-Associated CD4(+)CD8(hi) T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4(+)CD8(hi) phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4(+)CD8(hi) TIL have a similar expression of selected markers indicating that they rep(re)sent effector/memory alpha beta T-cell subset. Moreover, although TIL also contain CD4(-)CD8(hi) T-cells, only CD4(+)CD8(hi) TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.

Název v anglickém jazyce

The role of alpha beta T-cells in spontaneous regression of melanoma tumors in swine

Popis výsledku anglicky

Using a porcine model, we describe Melanoma-Associated CD4(+)CD8(hi) T-lymphocytes (MATL) in peripheral blood that increase during melanoma regression. These MATL possess the CD4(+)CD8(hi) phenotype and they have their direct counterparts in Tumor Infiltrating Lymphocytes (TIL) isolated from melanoma loci. Both MATL and CD4(+)CD8(hi) TIL have a similar expression of selected markers indicating that they rep(re)sent effector/memory alpha beta T-cell subset. Moreover, although TIL also contain CD4(-)CD8(hi) T-cells, only CD4(+)CD8(hi) TIL expand during melanoma regression. Importantly, TIL isolated from different pigs and different melanoma loci among the same pig have similar composition of CD4/CD8 subsets, indicating that the composition of the MATL and TIL compartment is identical. Analysis of sorted cells from regressing pigs revealed a unique MATL subpopulation with mono-specific T-cell receptor that was further analyzed by sequencing. These results indicate that pigs regressing melanomas possess a characteristic population of recirculating T-cells playing a role in tumor control and regression.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

ISSN

0145-305X

e-ISSN

1879-0089

Svazek periodika

92

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

60-68

Kód UT WoS článku

000458596200007

EID výsledku v databázi Scopus

2-s2.0-85056618764