Immunohistochemical Evidence of the Involvement of Natural Killer (CD161(+)) Cells in Spontaneous Regression of Lewis Rat Sarcoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00064211%3A_____%2F19%3AW0002048" target="_blank" >RIV/00064211:_____/19:W0002048 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985904:_____/19:00503687 RIV/60460709:41210/19:77995

Výsledek na webu

<a href="https://oadoi.org/10.21873/invivo.11437" target="_blank" >https://oadoi.org/10.21873/invivo.11437</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21873/invivo.11437" target="_blank" >10.21873/invivo.11437</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunohistochemical Evidence of the Involvement of Natural Killer (CD161(+)) Cells in Spontaneous Regression of Lewis Rat Sarcoma

Popis výsledku v původním jazyce

Background/Aim: Spontaneous regression (SR) of tumours is a rare phenomenon not yet fully understood. The aim of this study was to investigate immune cells infiltrating progressive and SR tumours in a Lewis rat sarcoma model. Materials and Methods: Rats were subcutaneously inoculated with rat sarcoma R5-28 (clone C4) cells. Developing tumours were obtained on day 42 and cryosections were immunohistochemically processed for detection of immune cells. Results: A high density of granulocytes was found in the necrotic areas of both progressive and SR tumours. CD4(+) cells and CD8(+) cells were rare and sparsely dispersed in the tumour tissue without clear difference between the two types of tumours. On the contrary, CD161(+) cells were abundant and evenly distributed in SR tumours, but these cells were very rare in progressive tumours. Conclusion: Based on the differences in number and distribution of the immune cell subpopulations, we believe that natural killer (CD161(+)) cells play a major role in the destruction of cancer cells during SR of tumours in this Lewis rat model.

Název v anglickém jazyce

Immunohistochemical Evidence of the Involvement of Natural Killer (CD161(+)) Cells in Spontaneous Regression of Lewis Rat Sarcoma

Popis výsledku anglicky

Background/Aim: Spontaneous regression (SR) of tumours is a rare phenomenon not yet fully understood. The aim of this study was to investigate immune cells infiltrating progressive and SR tumours in a Lewis rat sarcoma model. Materials and Methods: Rats were subcutaneously inoculated with rat sarcoma R5-28 (clone C4) cells. Developing tumours were obtained on day 42 and cryosections were immunohistochemically processed for detection of immune cells. Results: A high density of granulocytes was found in the necrotic areas of both progressive and SR tumours. CD4(+) cells and CD8(+) cells were rare and sparsely dispersed in the tumour tissue without clear difference between the two types of tumours. On the contrary, CD161(+) cells were abundant and evenly distributed in SR tumours, but these cells were very rare in progressive tumours. Conclusion: Based on the differences in number and distribution of the immune cell subpopulations, we believe that natural killer (CD161(+)) cells play a major role in the destruction of cancer cells during SR of tumours in this Lewis rat model.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

IN VIVO

ISSN

0258-851X

e-ISSN

1791-7549

Svazek periodika

33

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

6

Strana od-do

47-52

Kód UT WoS článku

000454333900007

EID výsledku v databázi Scopus

2-s2.0-85059241763