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3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098639%3A_____%2F16%3AN0000010" target="_blank" >RIV/00098639:_____/16:N0000010 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/62156489:43210/16:43909553 RIV/00216305:26620/16:PU120121

  • Výsledek na webu

    <a href="https://link.springer.com/article/10.1007%2Fs00706-016-1705-y" target="_blank" >https://link.springer.com/article/10.1007%2Fs00706-016-1705-y</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00706-016-1705-y" target="_blank" >10.1007/s00706-016-1705-y</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

  • Popis výsledku v původním jazyce

    We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (lambda = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile-butadiene-styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (lambda (exc) = 245 nm, lambda (em) = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 A degrees C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

  • Název v anglickém jazyce

    3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

  • Popis výsledku anglicky

    We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (lambda = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile-butadiene-styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (lambda (exc) = 245 nm, lambda (em) = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 A degrees C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10406 - Analytical chemistry

Návaznosti výsledku

  • Projekt

  • Návaznosti

    V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Ostatní

  • Rok uplatnění

    2016

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Monatshefte für Chemie - Chemical Monthly

  • ISSN

    0026-9247

  • e-ISSN

    1434-4475

  • Svazek periodika

    147

  • Číslo periodika v rámci svazku

    5

  • Stát vydavatele periodika

    AT - Rakouská republika

  • Počet stran výsledku

    8

  • Strana od-do

    873-880

  • Kód UT WoS článku

    000374172200005

  • EID výsledku v databázi Scopus