3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909553" target="_blank" >RIV/62156489:43210/16:43909553 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/16:PU120121 RIV/00098639:_____/16:N0000010

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00706-016-1705-y" target="_blank" >http://dx.doi.org/10.1007/s00706-016-1705-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-016-1705-y" target="_blank" >10.1007/s00706-016-1705-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Popis výsledku v původním jazyce

We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (lambda = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile-butadiene-styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (lambda (exc) = 245 nm, lambda (em) = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 A degrees C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

Název v anglickém jazyce

3D printed stratospheric probe as a platform for determination of DNA damage based on carbon quantum dots/DNA complex fluorescence increase

Popis výsledku anglicky

We present a utilization of carbon quantum dots (CQDs), passivated with polyethylene glycol as a fluorescent recognition probe for DNA damage. Synthesized CQDs were characterized in detail, using optical and electrochemical methods. Further, fluorescent behavior of CQDs was monitored in the presence of genomic DNA, isolated from Staphylococcus aureus. In laboratory conditions, after 30 min of exposure to UV irradiation (lambda = 254 nm), the DNA/CQDs complex significantly increased its fluorescence. Further, stratospheric probe was suggested and crafted by using technology of 3D printing (acrylonitrile-butadiene-styrene as a material). CQDs were exploited to evaluate the DNA damage in stratospheric conditions (up to 20,000 m) by determination of fluorescence increase (lambda (exc) = 245 nm, lambda (em) = 400 nm), together with other parameters (temperature, humidity, altitude, pressure, UV intensity, and X-ray irradiation). The obtained data showed that the sensor utilizing the DNA/CQDs was able to identify the DNA damage, together with external conditions. It was shown the proposed concept is able to operate at temperatures lower than -70 A degrees C. The proposed protocol may by applicable as a biosensor for long-term space missions, like international space station, missions to the Moon or Mars.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/LO1401" target="_blank" >LO1401: Interdisciplinární výzkum bezdrátových technologií</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte für Chemie - Chemical Monthly

ISSN

0026-9247

e-ISSN

—

Svazek periodika

147

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

873-880

Kód UT WoS článku

000374172200005

EID výsledku v databázi Scopus

2-s2.0-84960350670