Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F18%3AN0000029" target="_blank" >RIV/00098892:_____/18:N0000029 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15110/18:73587877 RIV/61989100:27240/18:10241688

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis

Popis výsledku v původním jazyce

Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis Anotace anglicky OBJECTIVE: There is no existing comprehensive report on the cellular composition of synovial fluids (SFs) from knee osteoarthritis (OA). We therefore aimed to characterise the immune cell composition in SFs from knee OA (KOA) and in subgroups according to gender. DESIGN: The immunophenotyping of monocyte/macrophage lineage cells, T and B cells, NK cells, neutrophils, dendritic and mast cells (MC) present in SFs from 53 patients (24 males/29 females) with KOA was performed using 6-colour flow cytometry. RESULTS: SFs from patients with OA contained 90 % hematopoietic cells. Lymphocytes were the predominant cell population (44.8 %) in the SFs of OA patients, with CD4+ T lymphocytes being more prevalent than CD8+ T cells (CD4+/CD8+ ratio = 1.3). Within the monocyte/macrophage lineage gating, monocytes accounted for 33.9 %, macrophages 14.8 %, myeloid dendritic cells 16.4 %. The rest of the hematopoietic cells were comprised of neutrophils (8 %), NK cells (3.8 %), T regulatory cells (1.2 %), plasmacytoid dendritic cells (1.1 %), mast cells (0.3 %). In OA females, a higher percentage of CD4+ T cells (P = 0.023), macrophages (P = 0.012), and a lower percentage of monocytes (P = 0.008) and CD8+ T cells (P = 0.002) were detected in comparison to OA males. CONCLUSIONS: Based on the immune cell composition of SFs, data mining analysis revealed distinct phenotypes (monocyte- and lymphocyte-predominant) within each gender group. This first study on the cellular complexity of SFs in KOA showed marked differences between male and female patients. The findings give a rational starting point for patient stratification according to their phenotypes, as is required for phenotype-specific treatment strategies.

Název v anglickém jazyce

Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis

Popis výsledku anglicky

Gender-related differences observed among immune cells in synovial fluid in knee osteoarthritis Anotace anglicky OBJECTIVE: There is no existing comprehensive report on the cellular composition of synovial fluids (SFs) from knee osteoarthritis (OA). We therefore aimed to characterise the immune cell composition in SFs from knee OA (KOA) and in subgroups according to gender. DESIGN: The immunophenotyping of monocyte/macrophage lineage cells, T and B cells, NK cells, neutrophils, dendritic and mast cells (MC) present in SFs from 53 patients (24 males/29 females) with KOA was performed using 6-colour flow cytometry. RESULTS: SFs from patients with OA contained 90 % hematopoietic cells. Lymphocytes were the predominant cell population (44.8 %) in the SFs of OA patients, with CD4+ T lymphocytes being more prevalent than CD8+ T cells (CD4+/CD8+ ratio = 1.3). Within the monocyte/macrophage lineage gating, monocytes accounted for 33.9 %, macrophages 14.8 %, myeloid dendritic cells 16.4 %. The rest of the hematopoietic cells were comprised of neutrophils (8 %), NK cells (3.8 %), T regulatory cells (1.2 %), plasmacytoid dendritic cells (1.1 %), mast cells (0.3 %). In OA females, a higher percentage of CD4+ T cells (P = 0.023), macrophages (P = 0.012), and a lower percentage of monocytes (P = 0.008) and CD8+ T cells (P = 0.002) were detected in comparison to OA males. CONCLUSIONS: Based on the immune cell composition of SFs, data mining analysis revealed distinct phenotypes (monocyte- and lymphocyte-predominant) within each gender group. This first study on the cellular complexity of SFs in KOA showed marked differences between male and female patients. The findings give a rational starting point for patient stratification according to their phenotypes, as is required for phenotype-specific treatment strategies.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30211 - Orthopaedics

Projekt

<a href="/cs/project/NV16-31852A" target="_blank" >NV16-31852A: Predikce rizika reoperace u pacientů s TEP kyčlí a kolen na základě imunogenetického vyšetření: vývoj kalkulátoru rizika pro rutinní klinické použití</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Osteoarthritis and Cartilage

ISSN

1063-4584

e-ISSN

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Svazek periodika

26

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

1247-1256

Kód UT WoS článku

000441526000013

EID výsledku v databázi Scopus

2-s2.0-85047333270