Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F23%3A73614585" target="_blank" >RIV/61989592:15110/23:73614585 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27240/23:10250541 RIV/00098892:_____/23:10157199

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1063458422008639?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1063458422008639?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.joca.2022.09.011" target="_blank" >10.1016/j.joca.2022.09.011</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection

Popis výsledku v původním jazyce

Objective: An increase in the number of neutrophils (NEUs) has long been associated with infections inthe knee joints; however, their impact on knee osteoarthritis (KOA) pathophysiology remains largelyunexplored.Design: This study compared the phenotypic and functional characteristics of synovial fluid (SF)-derivedNEUs in KOA and knee infection (INF).Results: KOA NEUs were characterised by a lower expression of CD11b, CD54, and CD64 and higherexpression of CD62L, TLR2, and TLR4 compared with INF NEUs. Except for CCL2, lower levels of inflammatory mediators and proteases were detected in KOA SF than in INF SF. Functionally, KOA NEUsdisplayed increased reactive oxygen species production and phagocytic activity compared with INF NEUs.Moreover, KOA and INF NEUs differed in cell sizes, histological characteristics of the surrounding synovialtissues, and their effects on the endothelial cells assessed by human umbilical vein endothelial cells.When KOA patients were subdivided based on the SF NEU abundance, patients with high NEUs (10%e60%) were characterised by i) elevated SF protein levels of TNF-a, IL-1RA, MMP-9, sTREM-1, VILIP-1 andii) lower CD54, CD64, TLR2 and TLR4 expression compared to patients with low NEUs (&lt;10%). Analysis ofpaired SF samples suggests that low or high NEU percentages, respectively, persist throughout the courseof disease.Conclusions: Our findings suggest that NEU may play a significant role in KOA pathophysiology. Furtherstudies should explore the mechanisms that contribute to the increased number of NEUs in SF and theclinical consequences of neutrophilic phenotype in KOA.

Název v anglickém jazyce

Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection

Popis výsledku anglicky

Objective: An increase in the number of neutrophils (NEUs) has long been associated with infections inthe knee joints; however, their impact on knee osteoarthritis (KOA) pathophysiology remains largelyunexplored.Design: This study compared the phenotypic and functional characteristics of synovial fluid (SF)-derivedNEUs in KOA and knee infection (INF).Results: KOA NEUs were characterised by a lower expression of CD11b, CD54, and CD64 and higherexpression of CD62L, TLR2, and TLR4 compared with INF NEUs. Except for CCL2, lower levels of inflammatory mediators and proteases were detected in KOA SF than in INF SF. Functionally, KOA NEUsdisplayed increased reactive oxygen species production and phagocytic activity compared with INF NEUs.Moreover, KOA and INF NEUs differed in cell sizes, histological characteristics of the surrounding synovialtissues, and their effects on the endothelial cells assessed by human umbilical vein endothelial cells.When KOA patients were subdivided based on the SF NEU abundance, patients with high NEUs (10%e60%) were characterised by i) elevated SF protein levels of TNF-a, IL-1RA, MMP-9, sTREM-1, VILIP-1 andii) lower CD54, CD64, TLR2 and TLR4 expression compared to patients with low NEUs (&lt;10%). Analysis ofpaired SF samples suggests that low or high NEU percentages, respectively, persist throughout the courseof disease.Conclusions: Our findings suggest that NEU may play a significant role in KOA pathophysiology. Furtherstudies should explore the mechanisms that contribute to the increased number of NEUs in SF and theclinical consequences of neutrophilic phenotype in KOA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

OSTEOARTHRITIS AND CARTILAGE

ISSN

1063-4584

e-ISSN

1522-9653

Svazek periodika

31

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

72-82

Kód UT WoS článku

000906534300001

EID výsledku v databázi Scopus

2-s2.0-85144588727