Multiparametric flow cytometry analysis of B cell trafficking enables the identification of immunoglobulin isotype-specific migration differences among EBV-infected and uninfected subpopulations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F19%3AN0000166" target="_blank" >RIV/00098892:_____/19:N0000166 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Multiparametric flow cytometry analysis of B cell trafficking enables the identification of immunoglobulin isotype-specific migration differences among EBV-infected and uninfected subpopulations

Popis výsledku v původním jazyce

13th World Immune Regulation Meeting, 06 - 09 April 2019, Davos, Switzerland – poster: Viral infection of cells modifies metabolism and functions including their maturation, phenotypes, and migration profiles. EBV infection targets predominantly B cells that differ from uninfected counterparts. Various EBV infection stages are associated with distinct expression of virus-specific antigens. Nevertheless, latently infected cells are difficult to identify by phenotypic markers. Combination of in situ hybridization using specific probes with the antibody staining of surface or intracellular markers by flow cytometry enables analysis of viral presence in millions of cells of several subsets. Moreover, the trafficking behavior could be characterized by staining for chemokine receptors, integrins or selectins. B cells were labeled for the presence of EBV (EBER1 probe) together with staining for B cell maturation markers (CD19, CD27, CD38, CD138), immunoglobulin isotypes (IgA, IgG, IgM, IgD), and surface homing molecules (a4b7 and a4b1, L-selectin,CCR5, CCR7, CCR9, and CCR10). Using these approaches we compared EBV-infected and uninfected B cells from healthy subjects. EBV-infected cells are predominantly IgA-positive memory B cells or plasmablasts. The expression of all chemokine receptors was increased in sIgG+ EBV+ B cell subpopulations, whereas sIgA+ EBV-infected cells increasingly expressed only CCR5 and CCR7. CCR10 was not changed and CCR9+ and α4β1+ subpopulation in sIgA+ EBV-infected cells was absent. The differences in the sIgA+ and sIgG+ subpopulations in EBV-infected B cells indicate distinct migration patterns in contrast to their healthy counterparts which could contribute in genetically predisposed subjects under various environmental factors to the development of symptoms as proposed for some autoimmune diseases and tumors.

Název v anglickém jazyce

Multiparametric flow cytometry analysis of B cell trafficking enables the identification of immunoglobulin isotype-specific migration differences among EBV-infected and uninfected subpopulations

Popis výsledku anglicky

13th World Immune Regulation Meeting, 06 - 09 April 2019, Davos, Switzerland – poster: Viral infection of cells modifies metabolism and functions including their maturation, phenotypes, and migration profiles. EBV infection targets predominantly B cells that differ from uninfected counterparts. Various EBV infection stages are associated with distinct expression of virus-specific antigens. Nevertheless, latently infected cells are difficult to identify by phenotypic markers. Combination of in situ hybridization using specific probes with the antibody staining of surface or intracellular markers by flow cytometry enables analysis of viral presence in millions of cells of several subsets. Moreover, the trafficking behavior could be characterized by staining for chemokine receptors, integrins or selectins. B cells were labeled for the presence of EBV (EBER1 probe) together with staining for B cell maturation markers (CD19, CD27, CD38, CD138), immunoglobulin isotypes (IgA, IgG, IgM, IgD), and surface homing molecules (a4b7 and a4b1, L-selectin,CCR5, CCR7, CCR9, and CCR10). Using these approaches we compared EBV-infected and uninfected B cells from healthy subjects. EBV-infected cells are predominantly IgA-positive memory B cells or plasmablasts. The expression of all chemokine receptors was increased in sIgG+ EBV+ B cell subpopulations, whereas sIgA+ EBV-infected cells increasingly expressed only CCR5 and CCR7. CCR10 was not changed and CCR9+ and α4β1+ subpopulation in sIgA+ EBV-infected cells was absent. The differences in the sIgA+ and sIgG+ subpopulations in EBV-infected B cells indicate distinct migration patterns in contrast to their healthy counterparts which could contribute in genetically predisposed subjects under various environmental factors to the development of symptoms as proposed for some autoimmune diseases and tumors.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30102 - Immunology

Projekt

<a href="/cs/project/NV15-33686A" target="_blank" >NV15-33686A: Studium vztahu mezi infekcí virem Epsteina a Barrové a rozvojem IgA nefropatie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů