Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F20%3A00537110" target="_blank" >RIV/61388971:_____/20:00537110 - isvavai.cz</a>

Výsledek na webu

<a href="https://biomed.papers.upol.cz/artkey/bio-202003-0004_multiparametric-flow-cytometry-analysis-of-peripheral-blood-b-cell-trafficking-differences-among-epstein-barr-v.php" target="_blank" >https://biomed.papers.upol.cz/artkey/bio-202003-0004_multiparametric-flow-cytometry-analysis-of-peripheral-blood-b-cell-trafficking-differences-among-epstein-barr-v.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2019.052" target="_blank" >10.5507/bp.2019.052</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

Popis výsledku v původním jazyce

Aims. Epstein-Barr virus (EBV) targets predominantly B cells and these cells could acquire new phenotype characteristics. Here we analyzed whether EBV-infected and -uninfected B cells from healthy subjects differ in proportion of dominant phenotypes, maturation stage, and homing receptors expression.nMethods. EBV-infected and -uninfected cells were identified by flow cytometry using fluorophore-labeled EBV RNA-specific DNA probes combined with fluorophore-labeled antibody to surface lineage markers, integrins, chemokine receptors, and immunoglobulin isotypes, including intracellular ones.nResults. Our results show that the trafficking characteristics of EBERpos B cells are distinct from EBERneg B cells with most dominant differences detected for alpha 4 beta 1 and alpha 4 beta 7 and CCR5 and CCR7. EBV-positive cells are predominantly memory IgM(+) B cells or plasmablasts/plasma cells (PB/PC) positive for IgA or less for IgM. In comparison to uninfected B cells, less EBV-positive B cells express alpha 4 beta 7 and almost no cells express alpha 4 beta 1. EBV-positive B cells contained significantly higher proportion of CCR5(+) and CCR7(+) cells in comparison to EBV-negative cells. In vitro exposure of blood mononuclear cells to pro-inflammatory cytokine IL-6 reduces population of EBV-positive B cell.nConclusion. Although EBV-infected B cells represent only a minor subpopulation, their atypical functions could contribute in predisposed person to development abnormities such as some autoimmune diseases or tumors. Using multi-parameter flow cytometry we characterized differences in migration of EBV-positive and -negative B cells of various maturation stage and isotype of produced antibodies particularly different targeting to mucosal tissues of gastrointestinal and respiratory tracts.

Název v anglickém jazyce

Multiparametric flow cytometry analysis of peripheral blood B cell trafficking differences among Epstein-Barr virus infected and uninfected subpopulations

Popis výsledku anglicky

Aims. Epstein-Barr virus (EBV) targets predominantly B cells and these cells could acquire new phenotype characteristics. Here we analyzed whether EBV-infected and -uninfected B cells from healthy subjects differ in proportion of dominant phenotypes, maturation stage, and homing receptors expression.nMethods. EBV-infected and -uninfected cells were identified by flow cytometry using fluorophore-labeled EBV RNA-specific DNA probes combined with fluorophore-labeled antibody to surface lineage markers, integrins, chemokine receptors, and immunoglobulin isotypes, including intracellular ones.nResults. Our results show that the trafficking characteristics of EBERpos B cells are distinct from EBERneg B cells with most dominant differences detected for alpha 4 beta 1 and alpha 4 beta 7 and CCR5 and CCR7. EBV-positive cells are predominantly memory IgM(+) B cells or plasmablasts/plasma cells (PB/PC) positive for IgA or less for IgM. In comparison to uninfected B cells, less EBV-positive B cells express alpha 4 beta 7 and almost no cells express alpha 4 beta 1. EBV-positive B cells contained significantly higher proportion of CCR5(+) and CCR7(+) cells in comparison to EBV-negative cells. In vitro exposure of blood mononuclear cells to pro-inflammatory cytokine IL-6 reduces population of EBV-positive B cell.nConclusion. Although EBV-infected B cells represent only a minor subpopulation, their atypical functions could contribute in predisposed person to development abnormities such as some autoimmune diseases or tumors. Using multi-parameter flow cytometry we characterized differences in migration of EBV-positive and -negative B cells of various maturation stage and isotype of produced antibodies particularly different targeting to mucosal tissues of gastrointestinal and respiratory tracts.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV15-33686A" target="_blank" >NV15-33686A: Studium vztahu mezi infekcí virem Epsteina a Barrové a rozvojem IgA nefropatie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOMEDICAL PAPERS-OLOMOUC

ISSN

1804-7521

e-ISSN

—

Svazek periodika

164

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

247-254

Kód UT WoS článku

000595645600004

EID výsledku v databázi Scopus

2-s2.0-85086109490