Hexahydrocannabinol intoxication – a challenge for toxicological analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00098892%3A_____%2F24%3A10159640" target="_blank" >RIV/00098892:_____/24:10159640 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Hexahydrocannabinol intoxication – a challenge for toxicological analysis

Popis výsledku v původním jazyce

Hexahydrocannabinol (HAC) is a semi-synthetic cannabinoid. A trace amount of HHC occurs naturally in cannabis plants (Cannabis). HHC is usually produced synthetically from cannabidiol, less commonly from delta-9-tetrahydrocannabinol (THC). HHC-containing products were sold in the Czech Republic without restrictions until early March 2024 as a substitute for cannabis and THC-containing products in several very attractive branded and unbranded products. In several cases, these products have caused severe user intoxication. From a clinical and a forensic point of view, it was necessary to develop selective and sensitive methods for the detection and determination of HHC in biological materials, namely blood serum and urine. GC-MS/MS and LC-HRMS methods were used to identify and determine HHC and its metabolites for clinical and forensic purposes. The developed GC-MS/MS method allows separation, identification, and sensitive determination of HHC with common cannabinoids such as THC, 11-OH-THC, and THC-COOH in blood serum after extraction and derivatization. GC-MS/MS also exhibit excellent selectivity for the resolution of A- and S-HHC isomers. The resolution of R- and S- isomers of HHC in blood serum is important because A-HHC is the significantly more physiologically active isomer, which is important from a clinical but mainly from a forensic point of view for the interpretation of the severity of intoxication and the impairment. LC-HRMS method allows fast and simple separation and identification of HHC and common metabolites in blood serum and urine. Protein precipitation was employed as the sample preparation method in this case. On the other hand, LC-HRMS did not allow the separation of HHC isomers. The advantage of the LC-HRMS method compared to the GC-MS/MS method is the possibility of identifying HHC metabolites even in cases where HHC is no longer present in detectable amounts in blood serum or urine.

Název v anglickém jazyce

Hexahydrocannabinol intoxication – a challenge for toxicological analysis

Popis výsledku anglicky

Hexahydrocannabinol (HAC) is a semi-synthetic cannabinoid. A trace amount of HHC occurs naturally in cannabis plants (Cannabis). HHC is usually produced synthetically from cannabidiol, less commonly from delta-9-tetrahydrocannabinol (THC). HHC-containing products were sold in the Czech Republic without restrictions until early March 2024 as a substitute for cannabis and THC-containing products in several very attractive branded and unbranded products. In several cases, these products have caused severe user intoxication. From a clinical and a forensic point of view, it was necessary to develop selective and sensitive methods for the detection and determination of HHC in biological materials, namely blood serum and urine. GC-MS/MS and LC-HRMS methods were used to identify and determine HHC and its metabolites for clinical and forensic purposes. The developed GC-MS/MS method allows separation, identification, and sensitive determination of HHC with common cannabinoids such as THC, 11-OH-THC, and THC-COOH in blood serum after extraction and derivatization. GC-MS/MS also exhibit excellent selectivity for the resolution of A- and S-HHC isomers. The resolution of R- and S- isomers of HHC in blood serum is important because A-HHC is the significantly more physiologically active isomer, which is important from a clinical but mainly from a forensic point of view for the interpretation of the severity of intoxication and the impairment. LC-HRMS method allows fast and simple separation and identification of HHC and common metabolites in blood serum and urine. Protein precipitation was employed as the sample preparation method in this case. On the other hand, LC-HRMS did not allow the separation of HHC isomers. The advantage of the LC-HRMS method compared to the GC-MS/MS method is the possibility of identifying HHC metabolites even in cases where HHC is no longer present in detectable amounts in blood serum or urine.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

30501 - Forensic science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Societatis Medicinae Legalis Slovacae

ISSN

1338-4589

e-ISSN

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Svazek periodika

14

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

6

Strana od-do

2-7

Kód UT WoS článku

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EID výsledku v databázi Scopus

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