Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F07%3A%230000222" target="_blank" >RIV/00159816:_____/07:#0000222 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp.

Popis výsledku v původním jazyce

A group of eight Gram-positive rods was isolated during a screening of Lactobacillus spp. in dental caries. All strains were isolated on MRS agar and revealed cell and colony morphology typical for the genus Lactobacillus. Phenotypical identification using conventional tests and API 50CH kit assigned all strains as Lactobacillus sp. biochemically close to Lactobacillus brevis. Further investigation using rep-PCR with the (GTG)5 primer separated analysed group into a homogeneous cluster clearly separatedfrom all lactobacilli included in the in-house CCM database but their (GTG)5-PCR fingerprints matched well Streptococcus mutans representatives. Further comparison of biochemical test results with characteristics stated for S. mutans clearly confirmed all strains as members of this species. Our results showed that S. mutans strains retrieved from dental caries can be misidentified as Lactobacillus sp. due to their good growth on MRS agar in typical Lactobacillus-like Gram-positive rods

Název v anglickém jazyce

Misidentification of Streptococcus mutans strains isolated from dental caries as Lactobacillus sp.

Popis výsledku anglicky

A group of eight Gram-positive rods was isolated during a screening of Lactobacillus spp. in dental caries. All strains were isolated on MRS agar and revealed cell and colony morphology typical for the genus Lactobacillus. Phenotypical identification using conventional tests and API 50CH kit assigned all strains as Lactobacillus sp. biochemically close to Lactobacillus brevis. Further investigation using rep-PCR with the (GTG)5 primer separated analysed group into a homogeneous cluster clearly separatedfrom all lactobacilli included in the in-house CCM database but their (GTG)5-PCR fingerprints matched well Streptococcus mutans representatives. Further comparison of biochemical test results with characteristics stated for S. mutans clearly confirmed all strains as members of this species. Our results showed that S. mutans strains retrieved from dental caries can be misidentified as Lactobacillus sp. due to their good growth on MRS agar in typical Lactobacillus-like Gram-positive rods

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FF - ORL, oftalmologie, stomatologie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0528" target="_blank" >1M0528: Stomatologické výzkumné centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

J Periodontal Res

ISSN

0022-3484

e-ISSN

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Svazek periodika

42

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DK - Dánské království

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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