Identification of lactobacilli isolated from dental caries of children

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00026373" target="_blank" >RIV/00216224:14310/08:00026373 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of lactobacilli isolated from dental caries of children

Popis výsledku v původním jazyce

The aim of this work was to analyse a group of 69 lactobacilli isolated from dental caries and root canals of the extracted teeth from early childhood caries (ECC) affected children up to age of six. Clinical samples obtained from individual patients were cultivated in a 5 % CO2 atmosphere on MRS agar and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization and identification of the isolates. Evaluation of obtained (GTG)5-PCR fingerprints assigned clearly 21 strains as Lactobacillus rhamnosus, 20 as Lactobacillus fermentum, 16 as Lactobacillus casei/paracasei, 7 as Lactobacillus salivarius, 4 as Lactobacillus plantarum and 1 as Lactobacillus gasseri.

Název v anglickém jazyce

Identification of lactobacilli isolated from dental caries of children

Popis výsledku anglicky

The aim of this work was to analyse a group of 69 lactobacilli isolated from dental caries and root canals of the extracted teeth from early childhood caries (ECC) affected children up to age of six. Clinical samples obtained from individual patients were cultivated in a 5 % CO2 atmosphere on MRS agar and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization and identification of the isolates. Evaluation of obtained (GTG)5-PCR fingerprints assigned clearly 21 strains as Lactobacillus rhamnosus, 20 as Lactobacillus fermentum, 16 as Lactobacillus casei/paracasei, 7 as Lactobacillus salivarius, 4 as Lactobacillus plantarum and 1 as Lactobacillus gasseri.

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/1M0528" target="_blank" >1M0528: Stomatologické výzkumné centrum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů