The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F13%3A00060688" target="_blank" >RIV/00159816:_____/13:00060688 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/13:00392737 RIV/00216305:26620/12:PU100939 RIV/00216224:14310/13:00081845

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12026-012-8379-2" target="_blank" >http://dx.doi.org/10.1007/s12026-012-8379-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12026-012-8379-2" target="_blank" >10.1007/s12026-012-8379-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin

Popis výsledku v původním jazyce

It is known that cells and organisms can indirectly "sense" changes in l-arginine availability via changes in the activity of various metabolic pathways. However, the mechanism(s) by which genes can be directly regulated by l-arginine in mammalian cellshave not yet been elucidated. We investigated the effect of l-arginine in the in vivo model of peritoneal inflammation in mice and in vitro in RAW 264.7 macrophages. A detailed analysis of basic physiological functions and selected intracellular signaling cascades revealed that l-arginine is crucial for the acceleration of macrophage activation by bacterial lipopolysaccharide. l-arginine increased the production of reactive oxygen species, nitric oxide, release of Ca2+, as well as inducible nitric oxidesynthase expression. Interestingly, the effect of l-arginine on macrophage activation was dependent on the phosphorylation of mitogen-activated protein kinases and activity of phospholipase C. In RAW 264.7 cells, l-arginine was shown to

Název v anglickém jazyce

The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin

Popis výsledku anglicky

It is known that cells and organisms can indirectly "sense" changes in l-arginine availability via changes in the activity of various metabolic pathways. However, the mechanism(s) by which genes can be directly regulated by l-arginine in mammalian cellshave not yet been elucidated. We investigated the effect of l-arginine in the in vivo model of peritoneal inflammation in mice and in vitro in RAW 264.7 macrophages. A detailed analysis of basic physiological functions and selected intracellular signaling cascades revealed that l-arginine is crucial for the acceleration of macrophage activation by bacterial lipopolysaccharide. l-arginine increased the production of reactive oxygen species, nitric oxide, release of Ca2+, as well as inducible nitric oxidesynthase expression. Interestingly, the effect of l-arginine on macrophage activation was dependent on the phosphorylation of mitogen-activated protein kinases and activity of phospholipase C. In RAW 264.7 cells, l-arginine was shown to

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Immunologic Research

ISSN

0257-277X

e-ISSN

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Svazek periodika

56

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

73-84

Kód UT WoS článku

000317849100007

EID výsledku v databázi Scopus

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