Stable Phenotype and Function of Immortalized Lin(-)Sca-1(+) Cardiac Progenitor Cells in Long-Term Culture: A Step Closer to Standardization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00061040" target="_blank" >RIV/00159816:_____/14:00061040 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1089/scd.2013.0305" target="_blank" >http://dx.doi.org/10.1089/scd.2013.0305</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/scd.2013.0305" target="_blank" >10.1089/scd.2013.0305</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Stable Phenotype and Function of Immortalized Lin(-)Sca-1(+) Cardiac Progenitor Cells in Long-Term Culture: A Step Closer to Standardization

Popis výsledku v původním jazyce

Putative cardiac progenitor cells (CPCs) have been identified in the myocardium and are regarded as promising candidates for cardiac cell-based therapies. Although two distinct populations of CPCs reached the clinical setting, more detailed studies are required to portray the optimal cell type and therapeutic setting to drive robust cell engraftment and cardiomyogenesis after injury. Owing to the scarcity of the CPCs and the need for reproducibility, the generation of faithful cellular models would facilitate this scrutiny. Here, we evaluate whether immortalized Lin(-)Sca-1(+) CPCs (iCPC(Sca-1)) represent their native-cell counterpart, thereby constituting a robust in vitro model system for standardized investigation in the cardiac field. iCPC(Sca-1) were established in vitro as plastic adherent cells endowed with robust self-renewal capacity while preserving a stable phenotype in long-term culture. iCPC(Sca-1) differentiated into cardiomyocytic-, endothelial-, and smooth muscle-like c

Název v anglickém jazyce

Stable Phenotype and Function of Immortalized Lin(-)Sca-1(+) Cardiac Progenitor Cells in Long-Term Culture: A Step Closer to Standardization

Popis výsledku anglicky

Putative cardiac progenitor cells (CPCs) have been identified in the myocardium and are regarded as promising candidates for cardiac cell-based therapies. Although two distinct populations of CPCs reached the clinical setting, more detailed studies are required to portray the optimal cell type and therapeutic setting to drive robust cell engraftment and cardiomyogenesis after injury. Owing to the scarcity of the CPCs and the need for reproducibility, the generation of faithful cellular models would facilitate this scrutiny. Here, we evaluate whether immortalized Lin(-)Sca-1(+) CPCs (iCPC(Sca-1)) represent their native-cell counterpart, thereby constituting a robust in vitro model system for standardized investigation in the cardiac field. iCPC(Sca-1) were established in vitro as plastic adherent cells endowed with robust self-renewal capacity while preserving a stable phenotype in long-term culture. iCPC(Sca-1) differentiated into cardiomyocytic-, endothelial-, and smooth muscle-like c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.100%2F02%2F0123" target="_blank" >ED1.100/02/0123: Fakultní nemocnice u sv. Anny v Brně - Mezinárodní centrum klinického výzkumu (FNUSA - ICRC)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Stem Cells and Development

ISSN

1547-3287

e-ISSN

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Svazek periodika

23

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

15

Strana od-do

1012-1026

Kód UT WoS článku

000334845500009

EID výsledku v databázi Scopus

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