Cell cycle and differentiation of Sca-1+ and Sca-1- hematopoietic stem and progenitor cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10382887" target="_blank" >RIV/00216208:11110/18:10382887 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.1080/15384101.2018.1502573" target="_blank" >https://doi.org/10.1080/15384101.2018.1502573</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15384101.2018.1502573" target="_blank" >10.1080/15384101.2018.1502573</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Cell cycle and differentiation of Sca-1+ and Sca-1- hematopoietic stem and progenitor cells
Popis výsledku v původním jazyce
Hematopoietic stem and progenitor cells (HSPCs) are crucial for lifelong blood cell production. We analyzed the cell cycle and cell production rate in HSPCs in murine hematopoiesis. The labeling of DNA-synthesizing cells by two thymidine analogues, optimized for in-vivo use, enabled determination of the cell cycle flow rate into G2-phase, the duration of S-phase and the average cell cycle time in Sca-1(+) and Sca-1(-) HSPCs. Determination of cells with 2n DNA content labeled in preceding S-phase was then used to establish the cell flow rates in G1-phase. Our measurements revealed a significant difference in how Sca-1(+) and Sca-1(-) myeloid progenitors self-renew and differentiate. Division of the Sca-1(+) progenitors led to loss of the Sca-1 marker in about half of newly produced cells, corresponding to asymmetric cell division. Sca-1(-) cells arising from cell division entered a new round of the cell cycle, corresponding to symmetric self-renewing cell division. The novel data also enabled the estimation of the cell production rates in Sca-1(+) and in three subtypes of Sca-1(-) HSPCs and revealed Sca-1 negative cells as the major amplification stage in the blood cell development.
Název v anglickém jazyce
Cell cycle and differentiation of Sca-1+ and Sca-1- hematopoietic stem and progenitor cells
Popis výsledku anglicky
Hematopoietic stem and progenitor cells (HSPCs) are crucial for lifelong blood cell production. We analyzed the cell cycle and cell production rate in HSPCs in murine hematopoiesis. The labeling of DNA-synthesizing cells by two thymidine analogues, optimized for in-vivo use, enabled determination of the cell cycle flow rate into G2-phase, the duration of S-phase and the average cell cycle time in Sca-1(+) and Sca-1(-) HSPCs. Determination of cells with 2n DNA content labeled in preceding S-phase was then used to establish the cell flow rates in G1-phase. Our measurements revealed a significant difference in how Sca-1(+) and Sca-1(-) myeloid progenitors self-renew and differentiate. Division of the Sca-1(+) progenitors led to loss of the Sca-1 marker in about half of newly produced cells, corresponding to asymmetric cell division. Sca-1(-) cells arising from cell division entered a new round of the cell cycle, corresponding to symmetric self-renewing cell division. The novel data also enabled the estimation of the cell production rates in Sca-1(+) and in three subtypes of Sca-1(-) HSPCs and revealed Sca-1 negative cells as the major amplification stage in the blood cell development.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10601 - Cell biology
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2018
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Cell Cycle
ISSN
1538-4101
e-ISSN
—
Svazek periodika
17
Číslo periodika v rámci svazku
16
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
13
Strana od-do
1979-1991
Kód UT WoS článku
000449552300004
EID výsledku v databázi Scopus
2-s2.0-85053411639