MECHANOSENSING PROTEINS YAP/TAZ DYNAMICALLY CONTROL CARDIAC CELL MATURATION AND FUNCTION BY SENSING ECM NANOTOPOGRAPHY

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00066037" target="_blank" >RIV/00159816:_____/16:00066037 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nanocon.eu/cz/o-konferenci-historie-sborniky-foto/2016/" target="_blank" >https://www.nanocon.eu/cz/o-konferenci-historie-sborniky-foto/2016/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

MECHANOSENSING PROTEINS YAP/TAZ DYNAMICALLY CONTROL CARDIAC CELL MATURATION AND FUNCTION BY SENSING ECM NANOTOPOGRAPHY

Popis výsledku v původním jazyce

The composition and arrangement of extracellular matrix (ECM) components in adult cardiac tissue plays a key role in the organization and alignment of synchronously and functionally beating cardiomyocytes. Following Heart failure (HF) - the end stage of cardiac pathologies in which cardiac muscle is not able to pump adequate blood to the organism - critical changes in mechanics and nanotopography occur along with the composition of cardiac ECM. Such modifications in ECM compliance and nanotopography - collectively defined as remodelling - are deemed to hinder local cell viability and function. At the moment, reliable in vitro models of the dynamic modifications occurring in vivo during cardiac remodelling process are missing. The Mechanosensing pathway Hippo acts through its effectors YAP/TAZ and is sensitive to changes in ECM mechanical properties. In the present study we demonstrate that, while being responsive to substrate compliance in a pathophysiological range (0.3-40 kPa), the nuclear localization and transcriptional activity of YAP/TAZ can be tuned by dynamical modifications of surface mechanics and nanotopography, as demonstrated by ad hoc developed thermo-responsive polymers displaying shape-memory properties.

Název v anglickém jazyce

MECHANOSENSING PROTEINS YAP/TAZ DYNAMICALLY CONTROL CARDIAC CELL MATURATION AND FUNCTION BY SENSING ECM NANOTOPOGRAPHY

Popis výsledku anglicky

The composition and arrangement of extracellular matrix (ECM) components in adult cardiac tissue plays a key role in the organization and alignment of synchronously and functionally beating cardiomyocytes. Following Heart failure (HF) - the end stage of cardiac pathologies in which cardiac muscle is not able to pump adequate blood to the organism - critical changes in mechanics and nanotopography occur along with the composition of cardiac ECM. Such modifications in ECM compliance and nanotopography - collectively defined as remodelling - are deemed to hinder local cell viability and function. At the moment, reliable in vitro models of the dynamic modifications occurring in vivo during cardiac remodelling process are missing. The Mechanosensing pathway Hippo acts through its effectors YAP/TAZ and is sensitive to changes in ECM mechanical properties. In the present study we demonstrate that, while being responsive to substrate compliance in a pathophysiological range (0.3-40 kPa), the nuclear localization and transcriptional activity of YAP/TAZ can be tuned by dynamical modifications of surface mechanics and nanotopography, as demonstrated by ad hoc developed thermo-responsive polymers displaying shape-memory properties.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

20501 - Materials engineering

Projekt

<a href="/cs/project/LQ1605" target="_blank" >LQ1605: Translační medicína</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

8TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH &amp; APPLICATION (NANOCON 2016)

ISBN

978-80-87294-71-0

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

419-423

Název nakladatele

TANGER LTD

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

19. 10. 2016

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000410656100073