Standardized hiPSCs culture – vitronectin coating for cGMP compliant protocol

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067632" target="_blank" >RIV/00159816:_____/17:00067632 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Standardized hiPSCs culture – vitronectin coating for cGMP compliant protocol

Popis výsledku v původním jazyce

Background. Human induced pluripotent stem cells (hiPSCs) hold great promise for disease modeling, drug screening, toxicology testing, regenerative medicine and more. An important task for stem cell research translation into the clinic is culturing cells in completely defined conditions. Currently, many researchers culture hiPSCs on a surface coated with Matrigel(R), poorly defined protein mixture of animal origin. Alternatively, vitronectin can be used. Methods. We cultured two hiPSCs lines in mTeSRTM1 cell culture medium on a vitronectin-coated surface and compared their pluripotent markers expression and differentiation capacity in vitro to cells cultured on Matrigel(R) coated surface. Results. hiPSCs cultured on both surfaces expressed pluripotent markers and differentiated into all three germ layers. Conclusion. In this study, we tested culture method in completely defined conditions, thus compatible with current good manufacturing practice (cGMP). We demonstrate that vitronectin is a suitable Matrigel(R) replacement

Název v anglickém jazyce

Standardized hiPSCs culture – vitronectin coating for cGMP compliant protocol

Popis výsledku anglicky

Background. Human induced pluripotent stem cells (hiPSCs) hold great promise for disease modeling, drug screening, toxicology testing, regenerative medicine and more. An important task for stem cell research translation into the clinic is culturing cells in completely defined conditions. Currently, many researchers culture hiPSCs on a surface coated with Matrigel(R), poorly defined protein mixture of animal origin. Alternatively, vitronectin can be used. Methods. We cultured two hiPSCs lines in mTeSRTM1 cell culture medium on a vitronectin-coated surface and compared their pluripotent markers expression and differentiation capacity in vitro to cells cultured on Matrigel(R) coated surface. Results. hiPSCs cultured on both surfaces expressed pluripotent markers and differentiated into all three germ layers. Conclusion. In this study, we tested culture method in completely defined conditions, thus compatible with current good manufacturing practice (cGMP). We demonstrate that vitronectin is a suitable Matrigel(R) replacement

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOMEDICAL PAPERS-OLOMOUC

ISSN

1213-8118

e-ISSN

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Svazek periodika

161

Číslo periodika v rámci svazku

S1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

"S25"-"S28"

Kód UT WoS článku

000428945000005

EID výsledku v databázi Scopus

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