Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita: a multicentre analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00068312" target="_blank" >RIV/00159816:_____/17:00068312 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/17:00099150

Výsledek na webu

<a href="http://dx.doi.org/10.1111/bjd.15800" target="_blank" >http://dx.doi.org/10.1111/bjd.15800</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/bjd.15800" target="_blank" >10.1111/bjd.15800</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita: a multicentre analysis

Popis výsledku v původním jazyce

BackgroundEpidermolysis bullosa acquisita (EBA) is a rare, potentially devastating autoimmune disease of the skin. IgG autoantibodies directed against type VII collagen (Col7), the major component of anchoring fibrils, induce skin fragility leading to cutaneous and mucocutaneous blister formation, which is mostly of a scarring phenotype. Thus, powerful and reproducible diagnostic assays are critical to establish the diagnosis of EBA early to avoid irreversible sequelae. ObjectivesThe present international, retrospective multicentre study included a large cohort of patients with EBA and evaluated the diagnostic power of four different diagnostic assays for the detection of anti-Col7 IgG autoantibodies. MethodsOverall, 95 EBA sera and 200 control sera consisting of 100 bullous pemphigoid sera, 50 pemphigus vulgaris sera and 50 sera of healthy controls were tested for anti-Col7 IgG autoantibodies using indirect immunofluorescence (IIF), two commercial enzyme-linked immunosorbent assay (ELISA) systems and Western blot (WB) analysis. EBA sera were taken from patients with positive direct immunofluorescence and IgG reactivity in at least one of the immunoserological assays (IIF, ELISA, WB). ResultsA Col7-NC1/NC2 ELISA (MBL, Nagoya, Japan) showed the highest sensitivity (979%), followed by a Col7-NC1 ELISA (Euroimmun, Lubeck, Germany) (895%), WB with Col7-NC1 (853%), and IIF on saline-split human skin (747%). The specificities of both ELISA systems were comparable (NC1 987%, NC1/NC2 993%). Furthermore, WB was more sensitive than IIF, which was more specific. ConclusionsThe two commercially available ELISA systems allow for a highly sensitive and specific diagnosis of EBA. The sensitivity of the Col7-NC1/NC2 ELISA is significantly higher compared with the ELISA based on the Col7-NC1 domain only. What&apos;s already known about this topic? ...

Název v anglickém jazyce

Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita: a multicentre analysis

Popis výsledku anglicky

BackgroundEpidermolysis bullosa acquisita (EBA) is a rare, potentially devastating autoimmune disease of the skin. IgG autoantibodies directed against type VII collagen (Col7), the major component of anchoring fibrils, induce skin fragility leading to cutaneous and mucocutaneous blister formation, which is mostly of a scarring phenotype. Thus, powerful and reproducible diagnostic assays are critical to establish the diagnosis of EBA early to avoid irreversible sequelae. ObjectivesThe present international, retrospective multicentre study included a large cohort of patients with EBA and evaluated the diagnostic power of four different diagnostic assays for the detection of anti-Col7 IgG autoantibodies. MethodsOverall, 95 EBA sera and 200 control sera consisting of 100 bullous pemphigoid sera, 50 pemphigus vulgaris sera and 50 sera of healthy controls were tested for anti-Col7 IgG autoantibodies using indirect immunofluorescence (IIF), two commercial enzyme-linked immunosorbent assay (ELISA) systems and Western blot (WB) analysis. EBA sera were taken from patients with positive direct immunofluorescence and IgG reactivity in at least one of the immunoserological assays (IIF, ELISA, WB). ResultsA Col7-NC1/NC2 ELISA (MBL, Nagoya, Japan) showed the highest sensitivity (979%), followed by a Col7-NC1 ELISA (Euroimmun, Lubeck, Germany) (895%), WB with Col7-NC1 (853%), and IIF on saline-split human skin (747%). The specificities of both ELISA systems were comparable (NC1 987%, NC1/NC2 993%). Furthermore, WB was more sensitive than IIF, which was more specific. ConclusionsThe two commercially available ELISA systems allow for a highly sensitive and specific diagnosis of EBA. The sensitivity of the Col7-NC1/NC2 ELISA is significantly higher compared with the ELISA based on the Col7-NC1 domain only. What&apos;s already known about this topic? ...

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30216 - Dermatology and venereal diseases

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

British Journal of Dermatology

ISSN

0007-0963

e-ISSN

—

Svazek periodika

177

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

1683-1692

Kód UT WoS článku

000419479300057

EID výsledku v databázi Scopus

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