Antimicrobial effect of commercial phage preparation Stafal (R) on biofilm and planktonic forms of methicillin-resistant Staphylococcus aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F19%3A00071015" target="_blank" >RIV/00159816:_____/19:00071015 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/19:00108452

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs12223-018-0622-3" target="_blank" >https://link.springer.com/article/10.1007%2Fs12223-018-0622-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-018-0622-3" target="_blank" >10.1007/s12223-018-0622-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antimicrobial effect of commercial phage preparation Stafal (R) on biofilm and planktonic forms of methicillin-resistant Staphylococcus aureus

Popis výsledku v původním jazyce

Staphylococcus aureus may be a highly virulent human pathogen, especially when it is able to form a biofilm, and it is resistant to antibiotic. Infections caused by these bacteria significantly affect morbidity and mortality, primarily in hospitalized patients. Treatment becomes more expensive, more toxic, and prolonged. This is the reason why research on alternative therapies should be one of the main priorities of medicine and biotechnology. A promising alternative treatment approach is bacteriophage therapy. The effect of the anti-staphylococcal bacteriophage preparation Stafal (R) on biofilm reduction was assessed on nine S. aureus strains using both sonication with subsequent quantification of surviving cells on the catheter surface and evaluation of biofilm reduction in microtiter plates. It was demonstrated that the bacteriophages destroy planktonic cells very effectively. However, to destroy cells embedded in the biofilm effectively requires a concentration at least ten times higher than that provided by the commercial preparation. The catheter disc method (CDM) allowed easier comparison of the effect on planktonic cells and cells in a biofilm than the microtiter plate (MTP) method.

Název v anglickém jazyce

Antimicrobial effect of commercial phage preparation Stafal (R) on biofilm and planktonic forms of methicillin-resistant Staphylococcus aureus

Popis výsledku anglicky

Staphylococcus aureus may be a highly virulent human pathogen, especially when it is able to form a biofilm, and it is resistant to antibiotic. Infections caused by these bacteria significantly affect morbidity and mortality, primarily in hospitalized patients. Treatment becomes more expensive, more toxic, and prolonged. This is the reason why research on alternative therapies should be one of the main priorities of medicine and biotechnology. A promising alternative treatment approach is bacteriophage therapy. The effect of the anti-staphylococcal bacteriophage preparation Stafal (R) on biofilm reduction was assessed on nine S. aureus strains using both sonication with subsequent quantification of surviving cells on the catheter surface and evaluation of biofilm reduction in microtiter plates. It was demonstrated that the bacteriophages destroy planktonic cells very effectively. However, to destroy cells embedded in the biofilm effectively requires a concentration at least ten times higher than that provided by the commercial preparation. The catheter disc method (CDM) allowed easier comparison of the effect on planktonic cells and cells in a biofilm than the microtiter plate (MTP) method.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV16-29916A" target="_blank" >NV16-29916A: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica (Praha)

ISSN

0015-5632

e-ISSN

—

Svazek periodika

64

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

121-126

Kód UT WoS článku

000455916000015

EID výsledku v databázi Scopus

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