A Simple Vacuum-Based Microfluidic Technique to Establish High-Throughput Organs-On-Chip and 3D Cell Cultures at the Microscale

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F19%3A00071017" target="_blank" >RIV/00159816:_____/19:00071017 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/admt.201800319" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/admt.201800319</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/admt.201800319" target="_blank" >10.1002/admt.201800319</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Simple Vacuum-Based Microfluidic Technique to Establish High-Throughput Organs-On-Chip and 3D Cell Cultures at the Microscale

Popis výsledku v původním jazyce

Microfluidic-based 3D cell culture and organs-on-chip have proved able to generate accurate in vitro models of human physiology. Their widespread application and adoption are however hampered by limited scalability and throughput. Here, a novel strategy is described to significantly enhance the throughput of microfluidic systems for 3D cell culture and organs-on-chips. A series of 3D culture chambers (up to 96 replicates) can be seeded with a single pipetting operation and a system of normally closed microfluidic valves ensures the resulting 3D microtissues are independent. Devices fabricated with this design principle are employed to perform 3D cultures of rat cardiac fibroblasts and profile two known drugs (doxorubicin, sotalol) in terms of cytotoxicity. In addition, human contractile cardiac microtissues is generated using iPSC-derived cardiac myocytes and functional assays on microtissues calcium transients after treatment with a known chronotropic drug (verapamil) are performed. The systems here described thus open up new perspective in the scalability of organs-on-chip and pave the way to multireplicate 3D cell cultures in microfluidics.

Název v anglickém jazyce

A Simple Vacuum-Based Microfluidic Technique to Establish High-Throughput Organs-On-Chip and 3D Cell Cultures at the Microscale

Popis výsledku anglicky

Microfluidic-based 3D cell culture and organs-on-chip have proved able to generate accurate in vitro models of human physiology. Their widespread application and adoption are however hampered by limited scalability and throughput. Here, a novel strategy is described to significantly enhance the throughput of microfluidic systems for 3D cell culture and organs-on-chips. A series of 3D culture chambers (up to 96 replicates) can be seeded with a single pipetting operation and a system of normally closed microfluidic valves ensures the resulting 3D microtissues are independent. Devices fabricated with this design principle are employed to perform 3D cultures of rat cardiac fibroblasts and profile two known drugs (doxorubicin, sotalol) in terms of cytotoxicity. In addition, human contractile cardiac microtissues is generated using iPSC-derived cardiac myocytes and functional assays on microtissues calcium transients after treatment with a known chronotropic drug (verapamil) are performed. The systems here described thus open up new perspective in the scalability of organs-on-chip and pave the way to multireplicate 3D cell cultures in microfluidics.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

20501 - Materials engineering

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced Materials Technologies

ISSN

2365-709X

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000455117500038

EID výsledku v databázi Scopus

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