Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00121562" target="_blank" >RIV/00216224:14310/21:00121562 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S092540052100232X#" target="_blank" >https://www.sciencedirect.com/science/article/pii/S092540052100232X#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2021.129664" target="_blank" >10.1016/j.snb.2021.129664</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices

Popis výsledku v původním jazyce

Luminescent chemical sensors have been proven to be a valuable asset in cell cultures and generally in microbiological studies. We present microfluidic cell culture devices with integrated optical chemical sensors. The integrated sensors offer the possibility to monitor dissolved oxygen and pH levels and enable the measurement of respiration and acidification rates. The cell metabolism was observed using this method by temporarily stopping the incubation flow, barely influencing the cell culture. A thermoplastic polymer is used as oxygen impermeable chip material to enable the measurement of respiration rates, which is not possible with widely used PDMS-based microfluidics. Hence, an innovative fast prototyping strategy is utilized to enable the replication of small series of thermoplastic microfluidic chips. We were able to demonstrate the suitability of this measurement method by monitoring the metabolic response of a human lung carcinoma epithelial-like cell line (A549) to the exposure of FCCP (Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone), a drug known for its up-regulating effect on respiration and acidification rates. This universal measurement approach can potentially be deployed in all sorts of microfluidic devices and help to retrieve valuable data from inside those systems beyond classical end-point detection methods. It therefore complements modern 3D cell cultures and organ-on-chip research with a powerful analytical technology for gathering information about ongoing cell metabolism.

Název v anglickém jazyce

Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices

Popis výsledku anglicky

Luminescent chemical sensors have been proven to be a valuable asset in cell cultures and generally in microbiological studies. We present microfluidic cell culture devices with integrated optical chemical sensors. The integrated sensors offer the possibility to monitor dissolved oxygen and pH levels and enable the measurement of respiration and acidification rates. The cell metabolism was observed using this method by temporarily stopping the incubation flow, barely influencing the cell culture. A thermoplastic polymer is used as oxygen impermeable chip material to enable the measurement of respiration rates, which is not possible with widely used PDMS-based microfluidics. Hence, an innovative fast prototyping strategy is utilized to enable the replication of small series of thermoplastic microfluidic chips. We were able to demonstrate the suitability of this measurement method by monitoring the metabolic response of a human lung carcinoma epithelial-like cell line (A549) to the exposure of FCCP (Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone), a drug known for its up-regulating effect on respiration and acidification rates. This universal measurement approach can potentially be deployed in all sorts of microfluidic devices and help to retrieve valuable data from inside those systems beyond classical end-point detection methods. It therefore complements modern 3D cell cultures and organ-on-chip research with a powerful analytical technology for gathering information about ongoing cell metabolism.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B: Chemical

ISSN

0925-4005

e-ISSN

—

Svazek periodika

334

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

„129664“

Kód UT WoS článku

000632948200003

EID výsledku v databázi Scopus

2-s2.0-85101348252