DEFORMATION RESPONSE OF POLYDIMETHYLSILOXANE SUBSTRATES SUBJECTED TO UNIAXIAL QUASI-STATIC LOADING

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F19%3A00072540" target="_blank" >RIV/00159816:_____/19:00072540 - isvavai.cz</a>

Výsledek na webu

<a href="https://ojs.cvut.cz/ojs/index.php/APP/article/view/6032" target="_blank" >https://ojs.cvut.cz/ojs/index.php/APP/article/view/6032</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14311/APP.2019.25.0079" target="_blank" >10.14311/APP.2019.25.0079</a>

Jazyk výsledku

angličtina

Název v původním jazyce

DEFORMATION RESPONSE OF POLYDIMETHYLSILOXANE SUBSTRATES SUBJECTED TO UNIAXIAL QUASI-STATIC LOADING

Popis výsledku v původním jazyce

To investigate cellular response of cardiomyocytes to substrate mechanics, biocompatible material with stiffness in physiological range is needed. PDMS based material is used for construction of microfluidic organ on chip devices for cell culture due to ease of device preparation, bonding, and possibility of surface functionalization. However it has stiffness orders of magnitude out of physiological range. Therefore, we adapted recently available protocol aiming to prepare substrates which offer stiffness in physiological range 5 MINUS SIGN 100 kPa using various mixtures of Sylgard. An in-house developed loading device with single micron position tracking accuracy and sub-micron position sensitivity was adapted for this experimental campaign. All batches of the samples were subjected to uniaxial loading. During quasi-static experiment the samples were compressed to minimally 40 % deformation. The results are represented in the form of stress-strain curves calculated from the acquired force and displacement data and elastic moduli are estimated.

Název v anglickém jazyce

DEFORMATION RESPONSE OF POLYDIMETHYLSILOXANE SUBSTRATES SUBJECTED TO UNIAXIAL QUASI-STATIC LOADING

Popis výsledku anglicky

To investigate cellular response of cardiomyocytes to substrate mechanics, biocompatible material with stiffness in physiological range is needed. PDMS based material is used for construction of microfluidic organ on chip devices for cell culture due to ease of device preparation, bonding, and possibility of surface functionalization. However it has stiffness orders of magnitude out of physiological range. Therefore, we adapted recently available protocol aiming to prepare substrates which offer stiffness in physiological range 5 MINUS SIGN 100 kPa using various mixtures of Sylgard. An in-house developed loading device with single micron position tracking accuracy and sub-micron position sensitivity was adapted for this experimental campaign. All batches of the samples were subjected to uniaxial loading. During quasi-static experiment the samples were compressed to minimally 40 % deformation. The results are represented in the form of stress-strain curves calculated from the acquired force and displacement data and elastic moduli are estimated.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Polytechnica CTU Proceedings

ISSN

2336-5382

e-ISSN

—

Svazek periodika

2019

Číslo periodika v rámci svazku

25

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

4

Strana od-do

79-82

Kód UT WoS článku

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EID výsledku v databázi Scopus

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