Geometric Control of Cell Behavior by Biomolecule Nanodistribution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F22%3A00077665" target="_blank" >RIV/00159816:_____/22:00077665 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/22:00127154

Výsledek na webu

<a href="https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00650" target="_blank" >https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00650</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsbiomaterials.2c00650" target="_blank" >10.1021/acsbiomaterials.2c00650</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Geometric Control of Cell Behavior by Biomolecule Nanodistribution

Popis výsledku v původním jazyce

Many dynamic interactions within the cell micro-environment modulate cell behavior and cell fate. However, the pathways and mechanisms behind cell-cell or cell-extracellular matrix interactions remain understudied, as they occur at a nanoscale level. Recent progress in nanotechnology allows for mimicking of the microenvironment at nanoscale in vitro; electron-beam lithography (EBL) is currently the most promising technique. Although this nanopatterning technique can generate nanostructures of good quality and resolution, it has resulted, thus far, in the production of only simple shapes (e.g., rectangles) over a relatively small area (100 x 100 mu m), leaving its potential in biological applications unfulfilled. Here, we used EBL for cell-interaction studies by coating cell-culture-relevant material with electron-conductive indium tin oxide, which formed nanopatterns of complex nanohexagonal structures over a large area (500 x 500 mu m). We confirmed the potential of EBL for use in cell-interaction studies by analyzing specific cell responses toward differentially distributed nanohexagons spaced at 1000, 500, and 250 nm. We found that our optimized technique of EBL with HaloTags enabled the investigation of broad changes to a cell-culture-relevant surface and can provide an understanding of cellular signaling mechanisms at a single-molecule level.

Název v anglickém jazyce

Geometric Control of Cell Behavior by Biomolecule Nanodistribution

Popis výsledku anglicky

Many dynamic interactions within the cell micro-environment modulate cell behavior and cell fate. However, the pathways and mechanisms behind cell-cell or cell-extracellular matrix interactions remain understudied, as they occur at a nanoscale level. Recent progress in nanotechnology allows for mimicking of the microenvironment at nanoscale in vitro; electron-beam lithography (EBL) is currently the most promising technique. Although this nanopatterning technique can generate nanostructures of good quality and resolution, it has resulted, thus far, in the production of only simple shapes (e.g., rectangles) over a relatively small area (100 x 100 mu m), leaving its potential in biological applications unfulfilled. Here, we used EBL for cell-interaction studies by coating cell-culture-relevant material with electron-conductive indium tin oxide, which formed nanopatterns of complex nanohexagonal structures over a large area (500 x 500 mu m). We confirmed the potential of EBL for use in cell-interaction studies by analyzing specific cell responses toward differentially distributed nanohexagons spaced at 1000, 500, and 250 nm. We found that our optimized technique of EBL with HaloTags enabled the investigation of broad changes to a cell-culture-relevant surface and can provide an understanding of cellular signaling mechanisms at a single-molecule level.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20900 - Industrial biotechnology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Biomaterials Science &amp; Engineering

ISSN

2373-9878

e-ISSN

—

Svazek periodika

8

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

4789-4806

Kód UT WoS článku

000892436600001

EID výsledku v databázi Scopus

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