A scoring system for histological evaluation of skin during Staphylococcus aureus infections

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F23%3A00079502" target="_blank" >RIV/00159816:_____/23:00079502 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11756-023-01567-4" target="_blank" >https://link.springer.com/article/10.1007/s11756-023-01567-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11756-023-01567-4" target="_blank" >10.1007/s11756-023-01567-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A scoring system for histological evaluation of skin during Staphylococcus aureus infections

Popis výsledku v původním jazyce

The histological evaluation of skin changes during Staphylococcus aureus (S. aureus) infection requires an exact approach with an accurate definition of individual changes and their parameters, including a classification score facilitating comparison among experiments. To propose methods and procedures for the evaluation of histological changes in skin infected by S. aureus strains in experimental pig models. Four pigs were included in the experiment. In the dorsal region of each pig, 10 wounds per pig (5 x 5 cm, 2 cm spacing) were created. Wounds in the individual pigs were inoculated with three different strains of methicillin- (oxacillin) and amoxicillin-resistant S. aureus at concentrations of 2 x 10(9)-5 x 10(9) CFU/ml. One pig was not inoculated and served as control. Subsequently, samples were collected from each wound on Days 3, 7, 10, ad 14 after inoculation processed by frozen section technique and the samples were stained with hematoxylin-eosin. Classification scores of selected parameters were determined and an average classification index was established at the end of the experiment. Histologically, granulomatous arrangement and superficial re-epithelialization were observed in the wounds of all animals. Compared to control, S. aureus infection documents slow healing of skin wounds with impaired epidermal formation (0.88 infected vs 1.45 control), re-epithelialization (1.25 vs 2.08), edema in the dermis (1.50 vs 2.67), inflammation in the dermis (0.50 vs 1.69), fibrosis in the dermis (4 vs 2.56), fibrosis in the hypodermis (4 vs 3.17) and, finally, necrosis in the dermis (1 vs 2.2). The results in the scores for scabs (1.25 vs 1.42), dermis (2.63 vs 2.67), and, finally, necrosis in the hypodermis (0.25 vs 0.75) did not differ among groups. The proposed methodological approach allowed quantification of histological changes and we recommend employing this scoring system in other studies evaluating the healing of skin wounds.

Název v anglickém jazyce

A scoring system for histological evaluation of skin during Staphylococcus aureus infections

Popis výsledku anglicky

The histological evaluation of skin changes during Staphylococcus aureus (S. aureus) infection requires an exact approach with an accurate definition of individual changes and their parameters, including a classification score facilitating comparison among experiments. To propose methods and procedures for the evaluation of histological changes in skin infected by S. aureus strains in experimental pig models. Four pigs were included in the experiment. In the dorsal region of each pig, 10 wounds per pig (5 x 5 cm, 2 cm spacing) were created. Wounds in the individual pigs were inoculated with three different strains of methicillin- (oxacillin) and amoxicillin-resistant S. aureus at concentrations of 2 x 10(9)-5 x 10(9) CFU/ml. One pig was not inoculated and served as control. Subsequently, samples were collected from each wound on Days 3, 7, 10, ad 14 after inoculation processed by frozen section technique and the samples were stained with hematoxylin-eosin. Classification scores of selected parameters were determined and an average classification index was established at the end of the experiment. Histologically, granulomatous arrangement and superficial re-epithelialization were observed in the wounds of all animals. Compared to control, S. aureus infection documents slow healing of skin wounds with impaired epidermal formation (0.88 infected vs 1.45 control), re-epithelialization (1.25 vs 2.08), edema in the dermis (1.50 vs 2.67), inflammation in the dermis (0.50 vs 1.69), fibrosis in the dermis (4 vs 2.56), fibrosis in the hypodermis (4 vs 3.17) and, finally, necrosis in the dermis (1 vs 2.2). The results in the scores for scabs (1.25 vs 1.42), dermis (2.63 vs 2.67), and, finally, necrosis in the hypodermis (0.25 vs 0.75) did not differ among groups. The proposed methodological approach allowed quantification of histological changes and we recommend employing this scoring system in other studies evaluating the healing of skin wounds.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biologia

ISSN

0006-3088

e-ISSN

1336-9563

Svazek periodika

79

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

9

Strana od-do

917-925

Kód UT WoS článku

001105224900001

EID výsledku v databázi Scopus

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