Cisplatin treatment reduces contraction to angiotensin II by altering expression of angiotensin II receptors: a pilot study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F23%3A00079641" target="_blank" >RIV/00159816:_____/23:00079641 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/23:00133476

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11010-023-04706-2" target="_blank" >https://link.springer.com/article/10.1007/s11010-023-04706-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-023-04706-2" target="_blank" >10.1007/s11010-023-04706-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cisplatin treatment reduces contraction to angiotensin II by altering expression of angiotensin II receptors: a pilot study

Popis výsledku v původním jazyce

The renin angiotensin system is a key regulator of blood pressure homeostasis. Angiotensin type 1 (AT(1)R) and 2 receptors (AT(2)R) have been investigated as targets for cisplatin-induced acute kidney injury; however, their therapeutic potential remains inconclusive. This pilot study aimed to determined the effect that acute cisplatin treatment had on angiotensin II (AngII)-induced contraction in blood vessels and expression profiles of AT(1)R and AT(2)R in mouse arteries and kidneys. Male C57BL/6 mice at 18 week of age (n=8) were treated with vehicle or bolus dose of cisplatin (12.5 mg/kg). Thoracic aorta (TA), adnominal aorta (AA), brachiocephalic arteries (BC), iliac arteries (IL) and kidneys were collected for isometric tension and immunohistochemistry analysis. Cisplatin treatment reduced IL contraction to AngII at all doses (p&lt;0.01, p&lt;0.001, p&lt;0.0001); however, AngII did not induce contraction in TA, AA or BC in either treatment group. Following cisplatin treatment, AT(1)R expression was significantly upregulated in the media of TA (p&lt;0.0001) and AA (p&lt;0.0001), and in the endothelium (p&lt;0.05) media (p&lt;0.0001) and adventitia (p&lt;0.01) of IL. Cisplatin treatment significantly reduced AT(2)R expression in the endothelium (p&lt;0.05) and media (p&lt;0.05) of TA. In renal tubules, both AT(1)R (p&lt;0.01) and AT(2)R (p&lt;0.05) were increased following cisplatin treatment. Herein, we report that cisplatin reduces AngII-mediated contraction in IL and may be explained by an absence of normal counterregulatory expression of AT(1)R and AT(2)R, indicating other factors are involved.

Název v anglickém jazyce

Cisplatin treatment reduces contraction to angiotensin II by altering expression of angiotensin II receptors: a pilot study

Popis výsledku anglicky

The renin angiotensin system is a key regulator of blood pressure homeostasis. Angiotensin type 1 (AT(1)R) and 2 receptors (AT(2)R) have been investigated as targets for cisplatin-induced acute kidney injury; however, their therapeutic potential remains inconclusive. This pilot study aimed to determined the effect that acute cisplatin treatment had on angiotensin II (AngII)-induced contraction in blood vessels and expression profiles of AT(1)R and AT(2)R in mouse arteries and kidneys. Male C57BL/6 mice at 18 week of age (n=8) were treated with vehicle or bolus dose of cisplatin (12.5 mg/kg). Thoracic aorta (TA), adnominal aorta (AA), brachiocephalic arteries (BC), iliac arteries (IL) and kidneys were collected for isometric tension and immunohistochemistry analysis. Cisplatin treatment reduced IL contraction to AngII at all doses (p&lt;0.01, p&lt;0.001, p&lt;0.0001); however, AngII did not induce contraction in TA, AA or BC in either treatment group. Following cisplatin treatment, AT(1)R expression was significantly upregulated in the media of TA (p&lt;0.0001) and AA (p&lt;0.0001), and in the endothelium (p&lt;0.05) media (p&lt;0.0001) and adventitia (p&lt;0.01) of IL. Cisplatin treatment significantly reduced AT(2)R expression in the endothelium (p&lt;0.05) and media (p&lt;0.05) of TA. In renal tubules, both AT(1)R (p&lt;0.01) and AT(2)R (p&lt;0.05) were increased following cisplatin treatment. Herein, we report that cisplatin reduces AngII-mediated contraction in IL and may be explained by an absence of normal counterregulatory expression of AT(1)R and AT(2)R, indicating other factors are involved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

1573-4919

Svazek periodika

478

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

2907-2916

Kód UT WoS článku

001099394000023

EID výsledku v databázi Scopus

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