Angiotensin II constricts mouse iliac arteries: possible mechanism for aortic aneurysms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F24%3A00079762" target="_blank" >RIV/00159816:_____/24:00079762 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/24:00135112

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11010-023-04724-0" target="_blank" >https://link.springer.com/article/10.1007/s11010-023-04724-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-023-04724-0" target="_blank" >10.1007/s11010-023-04724-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Angiotensin II constricts mouse iliac arteries: possible mechanism for aortic aneurysms

Popis výsledku v původním jazyce

Abdominal aortic aneurysms (AAA) result from maladaptive remodeling of the vascular wall and reduces structural integrity. Angiotensin II (AngII) infusion has become a standard laboratory model for studying AAA initiation and progression. We determined the different vasoactive responses of various mouse arteries to Ang II. Ex vivo isometric tension analysis was conducted on 18-week-old male C57BL/6 mice (n = 4) brachiocephalic arteries (BC), iliac arteries (IL), and abdominal (AA) and thoracic aorta (TA). Arterial rings were mounted between organ hooks, gently stretched and an AngII dose response was performed. Rings were placed in 4% paraformaldehyde for immunohistochemistry analysis to quantify peptide expression of angiotensin type 1 (AT(1)R) and 2 receptors (AT(2)R) in the endothelium, media, and adventitia. Results from this study demonstrated vasoconstriction responses in IL were significantly higher at all AngII doses when compared to BC, and TA and AA responses (maximum constriction-IL: 68.64 +/- 5.47% vs. BC: 1.96 +/- 1.00%; TA: 3.13 +/- 0.16% and AA: 2.75 +/- 1.77%, p &lt; 0.0001). Expression of AT(1)R was highest in the endothelium of IL (p &lt; 0.05) and in the media and (p &lt; 0.05) adventitia (p &lt; 0.05) of AA. In contrast, AT(2)R expression was highest in endothelium (p &lt; 0.05), media (p &lt; 0.01, p &lt; 0.05) and adventitia of TA. These results suggest that mouse arteries display different vasoactive responses to AngII, and the exaggerated response in IL arteries may play a role during AAA development.

Název v anglickém jazyce

Angiotensin II constricts mouse iliac arteries: possible mechanism for aortic aneurysms

Popis výsledku anglicky

Abdominal aortic aneurysms (AAA) result from maladaptive remodeling of the vascular wall and reduces structural integrity. Angiotensin II (AngII) infusion has become a standard laboratory model for studying AAA initiation and progression. We determined the different vasoactive responses of various mouse arteries to Ang II. Ex vivo isometric tension analysis was conducted on 18-week-old male C57BL/6 mice (n = 4) brachiocephalic arteries (BC), iliac arteries (IL), and abdominal (AA) and thoracic aorta (TA). Arterial rings were mounted between organ hooks, gently stretched and an AngII dose response was performed. Rings were placed in 4% paraformaldehyde for immunohistochemistry analysis to quantify peptide expression of angiotensin type 1 (AT(1)R) and 2 receptors (AT(2)R) in the endothelium, media, and adventitia. Results from this study demonstrated vasoconstriction responses in IL were significantly higher at all AngII doses when compared to BC, and TA and AA responses (maximum constriction-IL: 68.64 +/- 5.47% vs. BC: 1.96 +/- 1.00%; TA: 3.13 +/- 0.16% and AA: 2.75 +/- 1.77%, p &lt; 0.0001). Expression of AT(1)R was highest in the endothelium of IL (p &lt; 0.05) and in the media and (p &lt; 0.05) adventitia (p &lt; 0.05) of AA. In contrast, AT(2)R expression was highest in endothelium (p &lt; 0.05), media (p &lt; 0.01, p &lt; 0.05) and adventitia of TA. These results suggest that mouse arteries display different vasoactive responses to AngII, and the exaggerated response in IL arteries may play a role during AAA development.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

1573-4919

Svazek periodika

479

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

233-242

Kód UT WoS článku

000964127200002

EID výsledku v databázi Scopus

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