Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F24%3A00081022" target="_blank" >RIV/00159816:_____/24:00081022 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/24:00135617

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/abs/pii/S2772950824000621" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S2772950824000621</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioadv.2024.213819" target="_blank" >10.1016/j.bioadv.2024.213819</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

Popis výsledku v původním jazyce

Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from highmolecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young&apos;s moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.

Název v anglickém jazyce

Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells

Popis výsledku anglicky

Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from highmolecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young&apos;s moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomaterials Advances

ISSN

2772-9508

e-ISSN

2772-9508

Svazek periodika

159

Číslo periodika v rámci svazku

MAY 2024

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

213819

Kód UT WoS článku

001218651500001

EID výsledku v databázi Scopus

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